{"created":"2023-06-26T11:02:03.568298+00:00","id":2828,"links":{},"metadata":{"_buckets":{"deposit":"b1289f6e-e0ff-4a66-8b7c-6df839cdb732"},"_deposit":{"created_by":31,"id":"2828","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"2828"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002828","sets":["6:55"]},"author_link":["18112","18113","18111","18114","18115","18116"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-10-21","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"dfac056","bibliographic_titles":[{},{"bibliographic_title":"Microscopy","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Adaniya, Hidehito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Cheung, Martin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamashita, Masao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Taba, Seita"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Cassidy, Cathal"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shintake, Tsumoru"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"In this report, we applied annular bright-field (ABF) and annular dark-field (ADF) low-energy (30 keV) scanning transmission electron microscopy (STEM) imaging to a vitreous ice-embedded biological macromolecule, T4 phage, to investigate the applicability of these methods for morphological investigation and sample screening. Multiple camera lengths were examined to find the optimal acceptance angle for both modes. Image clarity differed substantially between the modes, with the presence of ice also strongly influencing the quality of acquired micrographs. In ADF mode, the proper discrimination of electrons scattered by the specimen from those scattered by the background ice was found to be difficult owing to the severe overlap of the scattered electrons. The resulting micrographs lacked clarity, and the ice-embedded phage particles could only be discerned after post-processing image adjustment. However, in ABF mode, despite similar overlapping of the scattered electrons, it was possible to assess the morphology and intactness of the specimen in the embedding ice, suggesting that this mode may find utility in low-energy cryo-STEM imaging methods.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press"},{"subitem_publisher":"The Japanese Society of Microscopy"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/36269113","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1093/jmicro/dfac056","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://academic.oup.com/jmicro/advance-article/doi/10.1093/jmicro/dfac056/6767311","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2022 The Author(s)"},{"subitem_rights":"This is a pre-copyedited, author-produced version of an article accepted for publication in Microscopy following peer review. The version of record Hidehito Adaniya, Martin Cheung, Masao Yamashita, Seita Taba, Cathal Cassidy, Tsumoru Shintake, Low-energy scanning transmission electron microscopy applied to ice-embedded biological macromolecules, Microscopy, 2022; is available online at: https://academic.oup.com/jmicro/advance-article/doi/10.1093/jmicro/dfac056/6767311."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2050-5698","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"2050-5701","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-10-21"}],"displaytype":"detail","filename":"Adaniya-2022-Low-energy scanning transmission (1)_VoR.pdf","filesize":[{"value":"2.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Adaniya-2022-Low-energy scanning transmission (1)_VoR","url":"https://oist.repo.nii.ac.jp/record/2828/files/Adaniya-2022-Low-energy scanning transmission (1)_VoR.pdf"},"version_id":"1c8ed7ad-457d-49a3-bf7f-88e82051f9b0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"low-energy","subitem_subject_scheme":"Other"},{"subitem_subject":"STEM imaging","subitem_subject_scheme":"Other"},{"subitem_subject":"cryo-EM","subitem_subject_scheme":"Other"},{"subitem_subject":"biological macromolecule","subitem_subject_scheme":"Other"},{"subitem_subject":"image contrast","subitem_subject_scheme":"Other"},{"subitem_subject":"electron scattering","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Low-energy scanning transmission electron microscopy applied to ice-embedded biological macromolecules","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Low-energy scanning transmission electron microscopy applied to ice-embedded biological macromolecules","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"31","path":["55"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-10-24"},"publish_date":"2022-10-24","publish_status":"0","recid":"2828","relation_version_is_last":true,"title":["Low-energy scanning transmission electron microscopy applied to ice-embedded biological macromolecules"],"weko_creator_id":"31","weko_shared_id":31},"updated":"2023-06-26T11:23:08.583399+00:00"}