{"created":"2023-06-26T11:00:05.325428+00:00","id":284,"links":{},"metadata":{"_buckets":{"deposit":"ae3cb063-7123-437c-a230-9192502f5a7c"},"_deposit":{"created_by":22,"id":"284","owners":[22],"pid":{"revision_id":0,"type":"depid","value":"284"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00000284","sets":["7:84"]},"author_link":[],"item_10006_creator_2":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"池上, 慶太","creatorNameLang":"ja"}]}]},"item_10006_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ikegami, Keita","creatorNameLang":"en"}]}]},"item_10006_date_granted_11":{"attribute_name":"Degree Conferral Date","attribute_value_mlt":[{"subitem_dategranted":"2018-02-09"}]},"item_10006_degree_grantor_9":{"attribute_name":"Degree Conferrral Institution","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"Okinawa Institute of Science and Technology Graduate University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"38005","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"Degree","attribute_value_mlt":[{"subitem_degreename":"Doctor of Philosophy"}]},"item_10006_description_7":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"The modification of embryogenesis in animals is an interesting research subject in evolutionary developmental biology. The phylotypic stage was originally described as a developmental period shared across vertebrates, where embryos resemble each other, and is represented by a narrowing waist of the developmental “hourglass” model. Recent studies of comprehensive gene expression have found support for this model in other metazoans, plants, and fungus. However, the existence of the phylotypic stage remains unresolved in deuterostomes. Thus, this study examines whether the phylotypic stage can be found across four deuterostome taxa, namely Ciona intestinalis from the Phylum Urochordata, Branchiostoma floridae from the Phylum Cephalochordata, Ptychodera flava from the Phylum Hemichordata, and Acanthaster planci from the Phylum Echinodermata. Comparison of gene expression profiles was carried out by microarray analysis of RNA across predetermined developmental time points. In this thesis, I developed a method of microarray probe design in A. planci, and proposed guidelines for the technology for use with other marine invertebrates. Next, I compared the gene expression profile with this microarray method. I found that the overall gene expression somewhat resembled each other in A. planci, P. flava and B. floridae, while C. intestinalis exhibited a unique pattern. The gene expression profiles of A. planci, P. flava and B. floridae showed narrowing waist-like pattern from the blastula to gastrula stages up to early larval stages; these stages were more conserved during embryogenesis in deuterostome taxa. However, I failed to find evidence for a typical vertebrate-like phylotypic stage in the four deuterostome taxa.","subitem_description_type":"Other"}]},"item_10006_dissertation_number_12":{"attribute_name":"Degree Referral Number","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第13号"}]},"item_10006_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15102/1394.00000256","subitem_identifier_reg_type":"JaLC"}]},"item_10006_rights_13":{"attribute_name":"Copyright Information","attribute_value_mlt":[{"subitem_rights":"© 2018 The Author"}]},"item_10006_text_24":{"attribute_name":"Exam Date","attribute_value_mlt":[{"subitem_text_value":"2017-08-03"}]},"item_10006_version_type_18":{"attribute_name":"Version Format","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-23"}],"displaytype":"detail","filename":"118.pdf","filesize":[{"value":"4.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Full-Text","objectType":"fulltext","url":"https://oist.repo.nii.ac.jp/record/284/files/118.pdf"},"version_id":"781de777-ab80-4cb9-929a-5aa18056b02d"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-23"}],"displaytype":"detail","filename":"doc(11).pdf","filesize":[{"value":"42.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Final Exam Abstract","objectType":"abstract","url":"https://oist.repo.nii.ac.jp/record/284/files/doc(11).pdf"},"version_id":"76606f70-8264-4658-8378-4a49e087eb31"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"新口動物における比較発生トランスクリプトミクスとファイロティピック段階に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"新口動物における比較発生トランスクリプトミクスとファイロティピック段階に関する研究","subitem_title_language":"ja"},{"subitem_title":"Comparative transcriptome analysis of basal deuterostomes and its implications for the phylotypic stage","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"22","path":["84"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-02-21"},"publish_date":"2018-02-21","publish_status":"0","recid":"284","relation_version_is_last":true,"title":["新口動物における比較発生トランスクリプトミクスとファイロティピック段階に関する研究"],"weko_creator_id":"22","weko_shared_id":-1},"updated":"2023-08-28T05:52:33.273554+00:00"}