@article{oai:oist.repo.nii.ac.jp:00002848,
 author = {Takahashi, Tohru M. and Hirano, Arisa and Kanda, Takeshi and Saito, Viviane M. and Ashitomi, Hiroto and Tanaka, Kazumasa Z. and Yokoshiki, Yasufumi and Masuda, Kosaku and Yanagisawa, Masashi and Vogt, Kaspar E. and Tokuda, Takashi and Sakurai, Takeshi},
 issue = {11},
 journal = {Cell Reports Methods},
 month = {Nov},
 note = {We recently determined that the excitatory manipulation of Qrfp-expressing neurons in the preoptic area of the hypothalamus (quiescence-inducing neurons [Q neurons]) induced a hibernation-like hypothermic/hypometabolic state (QIH) in mice. To control the QIH with a higher time resolution, we develop an optogenetic method using modified human opsin4 (OPN4; also known as melanopsin), a G protein-coupled-receptor-type blue-light photoreceptor. C-terminally truncated OPN4 (OPN4dC) stably and reproducibly induces QIH for at least 24 h by illumination with low-power light (3 μW, 473 nm laser) with high temporal resolution. The high sensitivity of OPN4dC allows us to transcranially stimulate Q neurons with blue-light-emitting diodes and non-invasively induce the QIH. OPN4dC-mediated QIH recapitulates the kinetics of the physiological changes observed in natural hibernation, revealing that Q neurons concurrently contribute to thermoregulation and cardiovascular function. This optogenetic method may facilitate identification of the neural mechanisms underlying long-term dormancy states such as sleep, daily torpor, and hibernation.},
 title = {Optogenetic induction of hibernation-like state with modified human Opsin4 in mice},
 volume = {2},
 year = {2022}
}