{"created":"2023-06-26T11:02:04.630048+00:00","id":2848,"links":{},"metadata":{"_buckets":{"deposit":"f3ee4983-d730-4015-9698-34ba3c428742"},"_deposit":{"created_by":31,"id":"2848","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"2848"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002848","sets":["6:187"]},"author_link":["18210","18199","18209","18208","18201","18203","18205","18200","18206","18204","18202","18207"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-11-21","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageStart":"100336","bibliographicVolumeNumber":"2","bibliographic_titles":[{},{"bibliographic_title":"Cell Reports Methods","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takahashi, Tohru M."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirano, Arisa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kanda, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saito, Viviane M."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ashitomi, Hiroto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Kazumasa Z."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yokoshiki, Yasufumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Masuda, Kosaku"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yanagisawa, Masashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Vogt, Kaspar E."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tokuda, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakurai, Takeshi"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"We recently determined that the excitatory manipulation of Qrfp-expressing neurons in the preoptic area of the hypothalamus (quiescence-inducing neurons [Q neurons]) induced a hibernation-like hypothermic/hypometabolic state (QIH) in mice. To control the QIH with a higher time resolution, we develop an optogenetic method using modified human opsin4 (OPN4; also known as melanopsin), a G protein-coupled-receptor-type blue-light photoreceptor. C-terminally truncated OPN4 (OPN4dC) stably and reproducibly induces QIH for at least 24 h by illumination with low-power light (3 μW, 473 nm laser) with high temporal resolution. The high sensitivity of OPN4dC allows us to transcranially stimulate Q neurons with blue-light-emitting diodes and non-invasively induce the QIH. OPN4dC-mediated QIH recapitulates the kinetics of the physiological changes observed in natural hibernation, revealing that Q neurons concurrently contribute to thermoregulation and cardiovascular function. This optogenetic method may facilitate identification of the neural mechanisms underlying long-term dormancy states such as sleep, daily torpor, and hibernation.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Cell Press"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1016/j.crmeth.2022.100336","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/pii/S2667237522002338","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2022 The Author(s)"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2667-2375","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-11-21"}],"displaytype":"detail","filename":"1-s2.0-S2667237522002338-main.pdf","filesize":[{"value":"24.6 MB"}],"format":"application/pdf","license_note":"CC BY 4.0\nCreative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"1-s2.0-S2667237522002338-main","url":"https://oist.repo.nii.ac.jp/record/2848/files/1-s2.0-S2667237522002338-main.pdf"},"version_id":"92c5445d-26d4-4962-abe9-8e5fd127eca0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"optogenetics","subitem_subject_scheme":"Other"},{"subitem_subject":"OPN4","subitem_subject_scheme":"Other"},{"subitem_subject":"hibernation","subitem_subject_scheme":"Other"},{"subitem_subject":"torpor","subitem_subject_scheme":"Other"},{"subitem_subject":"fiber-less optogenetics","subitem_subject_scheme":"Other"},{"subitem_subject":"melanopsin","subitem_subject_scheme":"Other"},{"subitem_subject":"QRFP","subitem_subject_scheme":"Other"},{"subitem_subject":"body temperature","subitem_subject_scheme":"Other"},{"subitem_subject":"GPCR","subitem_subject_scheme":"Other"},{"subitem_subject":"neuroscience","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Optogenetic induction of hibernation-like state with modified human Opsin4 in mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Optogenetic induction of hibernation-like state with modified human Opsin4 in mice","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"31","path":["187"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-11-17"},"publish_date":"2022-11-17","publish_status":"0","recid":"2848","relation_version_is_last":true,"title":["Optogenetic induction of hibernation-like state with modified human Opsin4 in mice"],"weko_creator_id":"31","weko_shared_id":31},"updated":"2023-06-26T11:22:31.940841+00:00"}