{"created":"2023-06-26T11:00:05.391068+00:00","id":285,"links":{},"metadata":{"_buckets":{"deposit":"bd9ddfd4-caa4-4b6e-8f11-139d3b7c95b5"},"_deposit":{"created_by":22,"id":"285","owners":[22],"pid":{"revision_id":0,"type":"depid","value":"285"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00000285","sets":["7:84"]},"author_link":[],"item_10006_creator_2":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"エスピナス, ニンニョ","creatorNameLang":"ja-Kana"}]}]},"item_10006_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Espinas, Nino","creatorNameLang":"en"}]}]},"item_10006_date_granted_11":{"attribute_name":"Degree Conferral Date","attribute_value_mlt":[{"subitem_dategranted":"2018-02-09"}]},"item_10006_degree_grantor_9":{"attribute_name":"Degree Conferrral Institution","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"Okinawa Institute of Science and Technology Graduate University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"38005","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"Degree","attribute_value_mlt":[{"subitem_degreename":"Doctor of Philosophy"}]},"item_10006_description_7":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Plant innate immunity against bacterial attacks is a two-tiered inducible system capable of defense responses at local and systemic areas. These systems are the PTI and ETI. During infection, PTI has the ability to recognize microbial signatures upon bacterial contact, while ETI recognizes microbial protein secretions called effectors delivered inside the cell. The activation of PTI and ETI confers systemic tissues of infected plants a broad-spectrum immunity against later pathogen attacks termed systemic acquired resistance (SAR). Defense priming is an adaptive component of SAR that regulates the molecular storage of defense memory for a more effective defense response.\n   The main aim of this work is finding a novel molecular defense signaling pathway that is controlled by acetylation at the infected (local defense) and systemic tissues (priming defense).\n   To investigate the role of histone acetyltransferase-dependent pathway in plant immunity, I have isolated transgenic and mutant lines of rCBP, [rice Cyclic adenosine monophosphate response element-binding protein (CREB) Binding Protein], under Nipponbare cultivar background using RNAi silencing and gRNA/Cas9-mediated genome editing. Animal CBP was initially described as both transcriptional coactivator and histone acetyltransferase. The rCBP-RNAi lines with mistargeting of the other members of CBP family are characterized by massive sterility and impairment of the number of effective grains. On the other hand, the CRISPR/Cas9 mutant lines have wild-type number of effective grains.\n   To profile the global acetylation of histone lysine-sites via rCBP, I performed mass spectrometry-based proteomics in data dependent acquisition (DDA) and parallel reaction monitoring (PRM) modes. My results showed that H3 lysine sites are possibly targeted by rCBP with very high acetylation specificity on H3K9.\n   To implicate the role of rCBP in rice innate immunity, I conducted a pathogenesis assay with bacterial pathogen, Pseudomonas syringiae pv. oryzae (Pso). Pathogenesis assay showed that rCBP-/- mutants are resistant to Pso infection compared to segregated wild-type control.\n   I also performed transcriptome analysis on local and systemic tissues with Pso to investigate the genome-wide effects of rCBP mutation and to identify factors with roles in both basal and systemic immune response. As a result, I have identified seven putative rCBPdependent transcriptional repressors that possibly explain the resistance phenotype of rCBP mutant lines.\n   Overall, these data preliminary indicate that rCBP is both a positive regulator of developmental processes and a negative regulator of rice immunity. These data also suggest that rCBP may execute this dual regulatory function either through H3K9ac and/or cotranscriptional activity on target gene loci.","subitem_description_type":"Other"}]},"item_10006_dissertation_number_12":{"attribute_name":"Degree Referral Number","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第12号"}]},"item_10006_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15102/1394.00000257","subitem_identifier_reg_type":"JaLC"}]},"item_10006_rights_13":{"attribute_name":"Copyright Information","attribute_value_mlt":[{"subitem_rights":"© 2018 The Author"}]},"item_10006_text_24":{"attribute_name":"Exam Date","attribute_value_mlt":[{"subitem_text_value":"2018-01-05"}]},"item_10006_version_type_18":{"attribute_name":"Version Format","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-09-01"}],"displaytype":"detail","filename":"128.pdf","filesize":[{"value":"2.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Full-Text","objectType":"fulltext","url":"https://oist.repo.nii.ac.jp/record/285/files/128.pdf"},"version_id":"4f4a0205-eced-4b26-a44f-14a76804e217"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-27"}],"displaytype":"detail","filename":"Abstract.pdf","filesize":[{"value":"94.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Abstract","objectType":"summary","url":"https://oist.repo.nii.ac.jp/record/285/files/Abstract.pdf"},"version_id":"fcf15ca3-9ec6-4c7b-b304-942e4d47bece"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-14"}],"displaytype":"detail","filename":"doc(9).pdf","filesize":[{"value":"42.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Final Exam Abstract","objectType":"abstract","url":"https://oist.repo.nii.ac.jp/record/285/files/doc(9).pdf"},"version_id":"6a888135-14d1-45ab-b077-db31b011de06"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"イネ自然免疫におけるrCBPの機能","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"イネ自然免疫におけるrCBPの機能","subitem_title_language":"ja"},{"subitem_title":"rCBP-dependent regulation in rice innate immunity","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"22","path":["84"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-02-21"},"publish_date":"2018-02-21","publish_status":"0","recid":"285","relation_version_is_last":true,"title":["イネ自然免疫におけるrCBPの機能"],"weko_creator_id":"22","weko_shared_id":-1},"updated":"2023-08-28T05:52:38.472659+00:00"}