{"created":"2023-06-26T11:02:06.552694+00:00","id":2881,"links":{},"metadata":{"_buckets":{"deposit":"9f704d1d-9671-41de-bbe2-25196214477f"},"_deposit":{"created_by":33,"id":"2881","owners":[33],"pid":{"revision_id":0,"type":"depid","value":"2881"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002881","sets":["6:51"]},"author_link":["18335","18332","18333","18334"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-09-16","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"101349","bibliographicVolumeNumber":"32","bibliographic_titles":[{},{"bibliographic_title":"Biochemistry and Biophysics Reports","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Adi, Tri Kustono"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujie, Manabu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Satoh, Nori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ueki, Tatsuya"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Ascidians accumulate extremely high levels of vanadium (V) in their blood cells. Several V-related proteins, including V-binding proteins (vanabins), have been isolated from V-accumulating ascidians. In this study, to obtain a deeper understanding of vanabins, we performed de novo transcriptome analysis of blood cells from a V-rich ascidian, Ascidia sydneiensis samea, and constructed a database containing 8532 predicted proteins. We found a novel vanabin with a unique acidic amino acid–rich C-terminal domain, designated VanabinX, in the database and studied it in detail. Reverse-transcription polymerase chain reaction analysis revealed that VanabinX was detected in all adult tissues examined, and was most prominent in blood cells and muscle tissue. We prepared recombinant proteins and performed immobilized metal ion affinity chromatography and a NADPH-coupled V(V)-reductase assay. VanabinX bound to metal ions, with increasing affinity for Cu(II) > Zn(II) > Co(II), but not to V(IV). VanabinX reduced V(V) to V(IV) at a rate of 0.170 μM per micoromolar protein within 30 min. The C-terminal acidic domain enhanced the reduction of V(V) by Vanabin2 to 1.3-fold and of VanabinX itself to 1.7-fold in trans mode. In summary, we constructed a protein database containing 8532 predicted proteins expressed in blood cells; among them, we discovered a novel vanabin, VanabinX, which enhances V reduction by vanabins.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/36147050","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1016/j.bbrep.2022.101349","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/pii/S2405580822001492?via%3Dihub","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2022 The Authors."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2405-5808","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-01-18"}],"displaytype":"detail","filename":"1-s2.0-S2405580822001492-main.pdf","filesize":[{"value":"4.4 MB"}],"format":"application/pdf","license_note":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (https://creativecommons.org/licenses/by-nc-nd/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"1-s2.0-S2405580822001492-main","url":"https://oist.repo.nii.ac.jp/record/2881/files/1-s2.0-S2405580822001492-main.pdf"},"version_id":"c3e406df-3b3b-4833-95e1-827d6dea91f4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Next-generation sequencing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Metal accumulation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Metal transporters","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Metal binding","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Enzyme kinetics","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The acidic amino acid-rich C-terminal domain of VanabinX enhances reductase activity, attaining 1.3- to 1.7-fold vanadium reduction","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The acidic amino acid-rich C-terminal domain of VanabinX enhances reductase activity, attaining 1.3- to 1.7-fold vanadium reduction","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"33","path":["51"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-01-18"},"publish_date":"2023-01-18","publish_status":"0","recid":"2881","relation_version_is_last":true,"title":["The acidic amino acid-rich C-terminal domain of VanabinX enhances reductase activity, attaining 1.3- to 1.7-fold vanadium reduction"],"weko_creator_id":"33","weko_shared_id":33},"updated":"2023-06-26T11:22:04.022699+00:00"}