{"created":"2023-06-26T11:00:12.190114+00:00","id":443,"links":{},"metadata":{"_buckets":{"deposit":"ad53adac-eedb-4110-bb0a-57858e398402"},"_deposit":{"created_by":27,"id":"443","owners":[27],"pid":{"revision_id":0,"type":"depid","value":"443"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00000443","sets":["6:51"]},"author_link":["1835","1832","1839","1836","1833","1838","1834","1837"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-09-15","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"17","bibliographicVolumeNumber":"3","bibliographic_titles":[{},{"bibliographic_title":"Zoological Letters","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sekigami, Yuka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Takuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Omi, Ai"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishitsuji, Koki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ikuta, Tetsuro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujiyama, Asao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Satoh, Noriyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saiga, Hidetoshi"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Background\nHox gene clusters with at least 13 paralog group (PG) members are common in vertebrate genomes and in that of amphioxus. Ascidians, which belong to the subphylum Tunicata (Urochordata), are phylogenetically positioned between vertebrates and amphioxus, and traditionally divided into two groups: the Pleurogona and the Enterogona. An enterogonan ascidian, Ciona intestinalis (Ci), possesses nine Hox genes localized on two chromosomes; thus, the Hox gene cluster is disintegrated. We investigated the Hox gene cluster of a pleurogonan ascidian, Halocynthia roretzi (Hr) to investigate whether Hox gene cluster disintegration is common among ascidians, and if so, how such disintegration occurred during ascidian or tunicate evolution.\nResults\nOur phylogenetic analysis reveals that the Hr Hox gene complement comprises nine members, including one with a relatively divergent Hox homeodomain sequence. Eight of nine Hr Hox genes were orthologous to Ci-Hox1, 2, 3, 4, 5, 10, 12 and 13. Following the phylogenetic classification into 13 PGs, we designated Hr Hox genes as Hox1, 2, 3, 4, 5, 10, 11/12/13.a, 11/12/13.b and HoxX. To address the chromosomal arrangement of the nine Hox genes, we performed two-color chromosomal fluorescent in situ hybridization, which revealed that the nine Hox genes are localized on a single chromosome in Hr, distinct from their arrangement in Ci. We further examined the order of the nine Hox genes on the chromosome by chromosome/scaffold walking. This analysis suggested a gene order of Hox1, 11/12/13.b, 11/12/13.a, 10, 5, X, followed by either Hox4, 3, 2 or Hox2, 3, 4 on the chromosome. Based on the present results and those previously reported in Ci, we discuss the establishment of the Hox gene complement and disintegration of Hox gene clusters during the course of ascidian or tunicate evolution.\nConclusions\nThe Hox gene cluster and the genome must have experienced extensive reorganization during the course of evolution from the ancestral tunicate to Hr and Ci. Nevertheless, some features are shared in Hox gene components and gene arrangement on the chromosomes, suggesting that Hox gene cluster disintegration in ascidians involved early events common to tunicates as well as later ascidian lineage-specific events.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Bio Med Central"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/28932414","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1186/s40851-017-0078-3","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://zoologicalletters.biomedcentral.com/articles/10.1186/s40851-017-0078-3#Abs1","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2017 The Author(s)."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2056-306X","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-07-24"}],"displaytype":"detail","filename":"Zoological letters 3-17 Sekigami et al 2017.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","license_note":"Article: Creative Commons Attribution 4.0 International\nhttps://creativecommons.org/licenses/by/4.0/\nData made available in this article: Creative Commons CC0 1.0 Universal\nhttps://creativecommons.org/publicdomain/zero/1.0/\n","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Zoological letters 3-17 Sekigami et al 2017","url":"https://oist.repo.nii.ac.jp/record/443/files/Zoological letters 3-17 Sekigami et al 2017.pdf"},"version_id":"fdca5a0d-4dd8-456d-bca7-5c108ef9c03e"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-07-24"}],"displaytype":"detail","filename":"40851_2017_78_MOESM1_ESM.pdf","filesize":[{"value":"42.7 kB"}],"format":"application/pdf","license_note":"Creative Commons CC0 1.0 Universal\nhttps://creativecommons.org/publicdomain/zero/1.0/","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"40851_2017_78_MOESM1_ESM","url":"https://oist.repo.nii.ac.jp/record/443/files/40851_2017_78_MOESM1_ESM.pdf"},"version_id":"5bc628b9-dc18-42c6-9ae1-ca36c34ac4bc"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-07-24"}],"displaytype":"detail","filename":"40851_2017_78_MOESM2_ESM.pdf","filesize":[{"value":"532.0 kB"}],"format":"application/pdf","license_note":"Creative Commons CC0 1.0 Universal\nhttps://creativecommons.org/publicdomain/zero/1.0/","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"40851_2017_78_MOESM2_ESM","url":"https://oist.repo.nii.ac.jp/record/443/files/40851_2017_78_MOESM2_ESM.pdf"},"version_id":"1c508d18-4ceb-4df5-841a-279bc49de4fb"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-07-24"}],"displaytype":"detail","filename":"40851_2017_78_MOESM3_ESM.pdf","filesize":[{"value":"252.0 kB"}],"format":"application/pdf","license_note":"Creative Commons CC0 1.0 Universal\nhttps://creativecommons.org/publicdomain/zero/1.0/","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"40851_2017_78_MOESM3_ESM","url":"https://oist.repo.nii.ac.jp/record/443/files/40851_2017_78_MOESM3_ESM.pdf"},"version_id":"dbe46f89-eb1c-4ade-a3f6-253f55db252c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Hox gene cluster of the ascidian, Halocynthia roretzi, reveals multiple ancient steps of cluster disintegration during ascidian evolution","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Hox gene cluster of the ascidian, Halocynthia roretzi, reveals multiple ancient steps of cluster disintegration during ascidian evolution","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"27","path":["51"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-07-24"},"publish_date":"2018-07-24","publish_status":"0","recid":"443","relation_version_is_last":true,"title":["Hox gene cluster of the ascidian, Halocynthia roretzi, reveals multiple ancient steps of cluster disintegration during ascidian evolution"],"weko_creator_id":"27","weko_shared_id":27},"updated":"2023-06-26T12:06:49.511300+00:00"}