{"created":"2023-06-26T11:00:21.852148+00:00","id":835,"links":{},"metadata":{"_buckets":{"deposit":"1d4c95cf-5d84-4ff3-9e52-c36f568c8d54"},"_deposit":{"created_by":26,"id":"835","owners":[26],"pid":{"revision_id":0,"type":"depid","value":"835"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00000835","sets":["6:52"]},"author_link":["4299","4298","4301","4300"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03-24","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"45166","bibliographicVolumeNumber":"7","bibliographic_titles":[{},{"bibliographic_title":"Scientific Reports","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Osabe, Kenji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Harukawa, Yoshiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miura, Saori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saze, Hidetoshi"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Defense mechanisms of plant genomes can epigenetically inactivate repetitive sequences and exogenous transgenes. Loss of mutant phenotypes in intronic T-DNA insertion lines by interaction with another T-DNA locus, termed T-DNA suppression, has been observed in Arabidopsis thaliana, although the molecular basis of establishment and maintenance of T-DNA suppression is poorly understood. Here we show that maintenance of T-DNA suppression requires heterochromatinisation of T-DNA sequences and the nuclear proteins, INCREASED IN BONSAI METHYLATION 2 (IBM2) and ENHANCED DOWNY MILDEW 2 (EDM2), which prevent ectopic 3′ end processing of mRNA in atypically long introns containing T-DNA sequences. Initiation of T-DNA suppression is mediated by the canonical RdDM pathway after hybridisation of two T-DNA strains, accompanied by DNA hypermethylation of T-DNA sequences in the F1 generation. Our results reveal the presence of a genome surveillance mechanism through genome hybridisation that masks repetitive DNAs intruding into transcription units.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Nature Research"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/28338020","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1038/srep45166","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.nature.com/articles/srep45166","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2017 The Author(s)."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-18"}],"displaytype":"detail","filename":"srep45166-1.pdf","filesize":[{"value":"2.2 MB"}],"format":"application/pdf","license_note":"Creative Commons Attribution 4.0 International\n(http://creativecommons.org/licenses/by/4.0/) ","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"srep45166-1","url":"https://oist.repo.nii.ac.jp/record/835/files/srep45166-1.pdf"},"version_id":"0a8973d1-dd7b-4167-8ccb-4c58550756d6"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-18"}],"displaytype":"detail","filename":"srep45166-s1.pdf","filesize":[{"value":"3.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"srep45166-s1","url":"https://oist.repo.nii.ac.jp/record/835/files/srep45166-s1.pdf"},"version_id":"00917ff7-0e5f-4fa4-86e0-02ceaba2c497"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Epigenetic Regulation of Intronic Transgenes in Arabidopsis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Epigenetic Regulation of Intronic Transgenes in Arabidopsis","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"26","path":["52"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-18"},"publish_date":"2019-03-18","publish_status":"0","recid":"835","relation_version_is_last":true,"title":["Epigenetic Regulation of Intronic Transgenes in Arabidopsis"],"weko_creator_id":"26","weko_shared_id":26},"updated":"2023-06-26T12:02:33.134428+00:00"}