@article{oai:oist.repo.nii.ac.jp:00000949,
 author = {Matsuura, Akihiro and Yoshimura, Ko and Kintsu, Hiroyuki and Atsumi, Takashi and Tsuchihashi, Yasushi and Takeuchi, Takeshi and Satoh, Noriyuki and Negishi, Lumi and Sakuda, Shohei and Asakura, Tomiko and Imura, Yuki and Yoshimura, Etsuro and Suzuki, Michio},
 issue = {2},
 journal = {Journal of Structural Biology},
 month = {Aug},
 note = {The pearl oyster, Pinctada fucata, is cultured for pearl production in Japan. The shell of the pearl oyster consists of calcium carbonate and a small amount of organic matrix. Despite many studies of the shell matrix proteins, the mechanism by which calcium elements are transported from the mantle to the shell remains unclear. Investigating the molecular mechanism of calcium transportation, we prepared artificial seawater with a high concentration of calcium ions (10ASW) to induce calcification in the pearl oyster. When pearl oysters were cultured in 10ASW, unusual nanoparticles were precipitated on the surface of the nacreous layer. SDS-PAGE and 2D-PAGE analyses revealed that some calcium-sensing proteins (Sarcoplasmic Ca-binding Protein (Pf-SCP) and Pf-filamin A) might be related to the synthesis of these nanoparticles. The recombinant proteins of Pf-SCP can bind to calcium ions and accumulate nanoparticles of calcium carbonate crystals. However, transcriptomic analysis of the pearl oysters grown in 10ASW showed that the matrix protein genes in the shell did not differ before and after treatment with 10ASW. These results suggest that, despite increasing calcium transportation to the shell, treatment with a high concentration of calcium ions does not induce formation of the organic framework in the shell microstructure. These findings offer meaningful insights into the transportation of calcium elements from the mantle to the shell.},
 pages = {240--249},
 title = {Structural and functional analyses of calcium ion response factors in the mantle of Pinctada fucata},
 volume = {204},
 year = {2018}
}