{"created":"2023-06-26T11:00:29.157520+00:00","id":988,"links":{},"metadata":{"_buckets":{"deposit":"0171ec41-eb5d-44e7-8bce-43f4cb5efa01"},"_deposit":{"created_by":28,"id":"988","owners":[28],"pid":{"revision_id":0,"type":"depid","value":"988"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00000988","sets":["6:51"]},"author_link":["5421","5415","5420","5416","5418","5419","5417"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-03-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageStart":"bio038422","bibliographicVolumeNumber":"8","bibliographic_titles":[{},{"bibliographic_title":"Biology Open","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kondo, Mariko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuo, Megumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Igarashi, Kento"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Haramoto, Yoshikazu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto, Takayoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yasuoka, Yuuri"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Taira, Masanori"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"hox genes are found as clusters in the genome in most bilaterians. The order of genes in the cluster is supposed to be correlated with the site of expression along the anterior-posterior body axis and the timing of expression during development, and these correlations are called spatial and temporal collinearity, respectively. Here we studied the expression dynamics of all hox genes of the diploid species Xenopus tropicalis in four Hox clusters (A-D) by analyzing high-temporal-resolution RNA-seq databases and the results showed that temporal collinearity is not supported, which is consistent with our previous data from allotetraploid X enopus laevis Because the temporal collinearity hypothesis implicitly assumes the collinear order of gene activation, not mRNA accumulation, we determined for the first time the timing of when new transcripts of hox genes are produced, by detecting pre-spliced RNA in whole embryos with reverse transcription and quantitative PCR (RT-qPCR) for all hoxa genes as well as several selected hoxb, hox c and hoxd genes. Our analyses showed that, coinciding with the RNA-seq results, hoxa genes started to be transcribed in a non-sequential order, and found that multiple genes start expression almost simultaneously or more posterior genes could be expressed earlier than anterior ones. This tendency was also found in hoxb and hoxc genes. These results suggest that temporal collinearity of hox genes is not held during early development of Xenopus.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"The Company of Biologists Ltd"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/30651235","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1242/bio.038422","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://creativecommons.org/licenses/by/4.0/"}]}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://bio.biologists.org/content/8/3/bio038422","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2019 The Company of Biologists Ltd. "}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2046-6390","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-04"}],"displaytype":"detail","filename":"Kondo-2019-De novo transcription of multiple H.pdf","filesize":[{"value":"2.0 MB"}],"format":"application/pdf","license_note":"Creative Commons​\nAttribution 4.0 International​\n(https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Kondo-2019-De novo transcription of multiple H","url":"https://oist.repo.nii.ac.jp/record/988/files/Kondo-2019-De novo transcription of multiple H.pdf"},"version_id":"0a82906a-837e-4c69-9477-057a94d07de5"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"De novo transcription of multiple Hox cluster genes takes place simultaneously in early Xenopus tropicalis embryos","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"De novo transcription of multiple Hox cluster genes takes place simultaneously in early Xenopus tropicalis embryos","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"28","path":["51"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-07-04"},"publish_date":"2019-07-04","publish_status":"0","recid":"988","relation_version_is_last":true,"title":["De novo transcription of multiple Hox cluster genes takes place simultaneously in early Xenopus tropicalis embryos"],"weko_creator_id":"28","weko_shared_id":28},"updated":"2023-06-26T11:59:04.991284+00:00"}