{"created":"2023-06-26T11:00:29.628923+00:00","id":995,"links":{},"metadata":{"_buckets":{"deposit":"f3addf27-923b-4665-9ca6-fff29d177625"},"_deposit":{"created_by":28,"id":"995","owners":[28],"pid":{"revision_id":0,"type":"depid","value":"995"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00000995","sets":["6:39"]},"author_link":["5464","5467","5468","5469","5470","5465","5466","5463"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-03-18","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"4829","bibliographicVolumeNumber":"9","bibliographic_titles":[{},{"bibliographic_title":"Scientific Reports","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Matsu-ura, Toru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shirakawa, Hideki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Kenichi G. N."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyamoto, Akitoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sugiura, Kotomi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Michikawa, Takayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kusumi, Akihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mikoshiba, Katsuhiko"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"In most species, fertilization induces Ca(2+) transients in the egg. In mammals, the Ca(2+) rises are triggered by phospholipase Czeta (PLCzeta) released from the sperm; IP3 generated by PLCzeta induces Ca(2+) release from the intracellular Ca(2+) store through IP3 receptor, termed IP3-induced Ca(2+) release. Here, we developed new fluorescent IP3 sensors (IRIS-2s) with the wider dynamic range and higher sensitivity (Kd = 0.047-1.7 muM) than that we developed previously. IRIS-2s employed green fluorescent protein and Halo-protein conjugated with the tetramethylrhodamine ligand as fluorescence resonance energy transfer (FRET) donor and acceptor, respectively. For simultaneous imaging of Ca(2+) and IP3, using IRIS-2s as the IP3 sensor, we developed a new single fluorophore Ca(2+) sensor protein, DYC3.60. With IRIS-2s and DYC3.60, we found that, right after fertilization, IP3 concentration ([IP3]) starts to increase before the onset of the first Ca(2+) wave. [IP3] stayed at the elevated level with small peaks followed after Ca(2+) spikes through Ca(2+) oscillations. We detected delays in the peak of [IP3] compared to the peak of each Ca(2+) spike, suggesting that Ca(2+)-induced regenerative IP3 production through PLC produces small [IP3] rises to maintain [IP3] over the basal level, which results in long lasting Ca(2+) oscillations in fertilized eggs.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Nature Research"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/30886280","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1038/s41598-019-40931-w","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://creativecommons.org/licenses/by/4.0/"}]}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.nature.com/articles/s41598-019-40931-w","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2019 The Author(s)."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-08"}],"displaytype":"detail","filename":"Matsu-Ura-2019-Dual-FRET imaging of IP3 and Ca.pdf","filesize":[{"value":"4.0 MB"}],"format":"application/pdf","license_note":"Creative Commons​\nAttribution 4.0 International​\n(https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Matsu-Ura-2019-Dual-FRET imaging of IP3 and Ca","url":"https://oist.repo.nii.ac.jp/record/995/files/Matsu-Ura-2019-Dual-FRET imaging of IP3 and Ca.pdf"},"version_id":"41d0d9da-0bd1-4300-bab1-0253b32d0900"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Dual-FRET imaging of IP3 and Ca2+ revealed Ca2+-induced IP3 production maintains long lasting Ca2+ oscillations in fertilized mouse eggs","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Dual-FRET imaging of IP3 and Ca2+ revealed Ca2+-induced IP3 production maintains long lasting Ca2+ oscillations in fertilized mouse eggs","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"28","path":["39"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-07-08"},"publish_date":"2019-07-08","publish_status":"0","recid":"995","relation_version_is_last":true,"title":["Dual-FRET imaging of IP3 and Ca2+ revealed Ca2+-induced IP3 production maintains long lasting Ca2+ oscillations in fertilized mouse eggs"],"weko_creator_id":"28","weko_shared_id":28},"updated":"2023-06-26T11:58:56.073672+00:00"}