@article{oai:oist.repo.nii.ac.jp:00000996, author = {Miyake, Takashi and Aihara, Natsuki and Maeda, Ken and Shinzato, Chuya and Koyanagi, Ryo and Kobayashi, Hirozumi and Yamahira, Kazunori}, issue = {1}, journal = {Scientific Reports}, month = {Mar}, note = {The mosquito, Aedes baisasi, which inhabits brackish mangrove swamps, is known to feed on fish. However, its host assemblage has not been investigated at the species level. We amplified and sequenced the cytochrome oxidase subunit I barcoding regions as well as some other regions from blood-fed females to identify host assemblages in the natural populations from four islands in the Ryukyu Archipelago. Hosts were identified from 230 females. We identified 15 host fish species belonging to eight families and four orders. Contrary to expectations from previous observations, mudskippers were detected from only 3% of blood-engorged females. The dominant host was a four-eyed sleeper, Bostrychus sinensis (Butidae, Gobiiformes), in Iriomote-jima Island (61%), while it was a snake eel, Pisodonophis boro (Ophichthidae, Anguilliformes), in Amami-oshima and Okinawa-jima islands (78% and 79%, respectively). Most of the identified hosts were known as air-breathing or amphibious fishes that inhabit mangroves or lagoons. Our results suggest that A. baisasi females locate the bloodmeal hosts within the mangrove forests and sometimes in the adjacent lagoons and land on the surface of available amphibious or other air-breathing fishes exposed in the air to feed on their blood.}, title = {Bloodmeal host identification with inferences to feeding habits of a fish-fed mosquito, Aedes baisasi}, volume = {9}, year = {2019} }