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Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs
https://oist.repo.nii.ac.jp/records/293
https://oist.repo.nii.ac.jp/records/293dee7306c-c005-469f-8c4f-790e8e85c596
名前 / ファイル | ライセンス | アクション |
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1183.full (3.1 MB)
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Creative Commons Attribution–Noncommercial–Share Alike 4.0 International
(https://creativecommons.org/licenses/by-nc-sa/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-02-27 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Kinoshita, Masanao
× Kinoshita, Masanao× Suzuki, Kenichi G.N.× Matsumori, Nobuaki× Takada, Misa× Ano, Hikaru× Morigaki, Kenichi× Abe, Mitsuhiro× Makino, Asami× Kobayashi, Toshihide× Hirosawa, Koichiro M.× Fujiwara, Takahiro K.× Kusumi, Akihiro× Murata, Michio |
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書誌情報 |
en : The Journal of Cell Biology 巻 216, 号 4, p. 1183-1204, 発行日 2017-03-22 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Sphingomyelin (SM) has been proposed to form cholesterol-dependent raft domains and sphingolipid domains in the plasma membrane (PM). How SM contributes to the formation and function of these domains remains unknown, primarily because of the scarcity of suitable fluorescent SM analogs. We developed new fluorescent SM analogs by conjugating a hydrophilic fluorophore to the SM choline headgroup without eliminating its positive charge, via a hydrophilic nonaethylene glycol linker. The new analogs behaved similarly to the native SM in terms of their partitioning behaviors in artificial liquid order-disorder phase-separated membranes and detergent-resistant PM preparations. Single fluorescent molecule tracking in the live-cell PM revealed that they indirectly interact with each other in cholesterol- and sphingosine backbone–dependent manners, and that, for ∼10–50 ms, they undergo transient colocalization-codiffusion with a glycosylphosphatidylinositol (GPI)-anchored protein, CD59 (in monomers, transient-dimer rafts, and clusters), in CD59-oligomer size–, cholesterol-, and GPI anchoring–dependent manners. These results suggest that SM continually and rapidly exchanges between CD59-associated raft domains and the bulk PM. | |||||
出版者 | ||||||
出版者 | Rockefeller University Press | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0021-9525 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1540-8140 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/28330937 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1083/jcb.201607086 | |||||
権利 | ||||||
権利情報 | © 2017 Kinoshita et al. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://jcb.rupress.org/content/216/4/1183 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |