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Intracellular Nanomaterial Delivery via Spiral Hydroporation
https://oist.repo.nii.ac.jp/records/1444
https://oist.repo.nii.ac.jp/records/1444fd402ba4-0d2c-4cfd-bd7b-2c5100add3c6
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Kang-2020-Intracellular Nanomaterial Delivery (8.5 MB)
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (https://creativecommons.org/licenses/by-nc-nd/4.0/)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-05-01 | |||||
| タイトル | ||||||
| タイトル | Intracellular Nanomaterial Delivery via Spiral Hydroporation | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者（英） |
Kang, GeoumYoung
× Kang, GeoumYoung× Carlson, Daniel W.× Kang, Tae Ho× Lee, Seungki× Haward, Simon J.× Choi, Inhee× Shen, Amy Q.× Chung, Aram J. |
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| 書誌情報 |
en : ACS Nano 巻 14, 号 3, p. 3048-3058, 発行日 2020-02-18 |
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| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | In recent nanobiotechnology developments, a wide variety of functional nanomaterials and engineered biomolecules have been created, and these have numerous applications in cell biology. For these nanomaterials to fulfill their promises completely, they must be able to reach their biological targets at the subcellular level and with a high level of specificity. Traditionally, either nanocarrier- or membrane disruption-based method has been used to deliver nanomaterials inside cells; however, these methods are suboptimal due to their toxicity, inconsistent delivery, and low throughput, and they are also labor intensive and time-consuming, highlighting the need for development of a next-generation, intracellular delivery system. This study reports on the development of an intracellular nanomaterial delivery platform, based on unexpected cell-deformation phenomena via spiral vortex and vortex breakdown exerted in the cross- and T-junctions at moderate Reynolds numbers. These vortex-induced cell deformation and sequential restoration processes open cell membranes transiently, allowing effective and robust intracellular delivery of nanomaterials in a single step without the aid of carriers or external apparatus. By using the platform described here (termed spiral hydroporator), we demonstrate the delivery of different nanomaterials, including gold nanoparticles (200 nm diameter), functional mesoporous silica nanoparticles (150 nm diameter), dextran (hydrodynamic diameters between 2–55 nm), and mRNA, into different cell types. We demonstrate here that the system is highly efficient (up to 96.5%) with high throughput (up to 1 × 106 cells/min) and rapid delivery (∼1 min) while maintaining high levels of cell viability (up to 94%). | |||||
| 出版者 | ||||||
| 出版者 | American Chemical Society | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1936-0851 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1936-086X | |||||
| PubMed番号 | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | info:pmid/32069037 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1021/acsnano.9b07930 | |||||
| 権利 | ||||||
| 権利情報 | © 2020 American Chemical Society | |||||
| 権利 | ||||||
| 権利情報 | ACS AuthorChoice with CC-BY-NC-ND | |||||
| 情報源 | ||||||
| 関連名称 | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://pubs.acs.org/doi/10.1021/acsnano.9b07930 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
