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Monitoring Protein Denaturation of Egg White Using Passive Microwave Radiometry (MWR)
https://oist.repo.nii.ac.jp/records/2683
https://oist.repo.nii.ac.jp/records/26837a4cf941-9a8a-4dd5-9760-4ceda241460d
名前 / ファイル | ライセンス | アクション |
---|---|---|
Goryanin-2022-Monitoring Protein Denaturation (4.4 MB)
|
CC BY 4.0
Creative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2022-07-06 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Monitoring Protein Denaturation of Egg White Using Passive Microwave Radiometry (MWR) | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | microwave radiometry | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | passive microwave emission | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | protein denaturation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | enzyme assays | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | drug discovery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | brain trauma | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Goryanin, Igor
× Goryanin, Igor× Ovchinnikov, Lev× Vesnin, Sergey× Ivanov, Yuri |
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書誌情報 |
en : Diagnostics 巻 12, 号 6, p. 1498, 発行日 2022-06-19 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Passive microwave radiometry (MWR) is a measurement technique based on the detection of passive radiation in the microwave spectrum of different objects. When in equilibrium, this radiation is known to be proportional to the thermodynamic temperature of an emitting body. We hypothesize that living systems feature other mechanisms of emission that are based on protein unfolding and water rotational transitions. To understand the nature of these emissions, microwave radiometry was used in several in vitro experiments. In our study, we performed pilot measurements of microwave emissions from egg whites during denaturation induced by ethanol. Egg whites comprise 10% proteins, such as albumins, mucoproteins, and globulins. We observed a novel phenomenon: microwave emissions changed without a corresponding change in the water’s thermodynamic temperature. We also found striking differences between microwave emissions and thermodynamic temperature kinetics. Therefore, we hypothesize that these two processes are unrelated, contrary to what was thought before. It is known that some pathologies such as stroke or brain trauma feature increased microwave emissions. We hypothesize that this phenomenon originates from protein denaturation and is not related to the thermodynamic temperature. As such, our findings could explain the reason for the increase in microwave emissions after trauma and post mortem for the first time. These findings could be used for the development of novel diagnostics methods. The MWR method is inexpensive and does not require fluorescent or radioactive labels. It can be used in different areas of basic and applied pharmaceutical research, including in kinetics studies in biomedicine. | |||||
出版者 | ||||||
出版者 | MDPI | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2075-4418 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/35741308 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.3390/diagnostics12061498 | |||||
権利 | ||||||
権利情報 | © 2022 The Author(s). | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.mdpi.com/2075-4418/12/6/1498 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |