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Spatial separation of FtsZ and FtsN during cell division
https://oist.repo.nii.ac.jp/records/411
https://oist.repo.nii.ac.jp/records/411df3320a4-818c-49ba-9a21-f2c51be1edfb
名前 / ファイル | ライセンス | アクション |
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Accepted version (13.2 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-11-30 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Spatial separation of FtsZ and FtsN during cell division | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Söderström, Bill
× Söderström, Bill× Chan, Helena× Shilling, Patrick J.× Skoglund, Ulf× Daley, Daniel O. |
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書誌情報 |
en : Molecular Microbiology 巻 107, 号 3, p. 387-401, 発行日 2017-12-19 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The division of Escherichia coli is mediated by a collection of some 34 different proteins that are recruited to the division septum and are thought to assemble into a macromolecular complex known as ‘the divisome’. Herein, we have endeavored to better understand the structure of the divisome by imaging two of its core components; FtsZ and FtsN. Super resolution microscopy (SIM and gSTED) indicated that both proteins are localized in large assemblies, which are distributed around the division septum (i.e., forming a discontinuous ring). Although the rings had similar radii prior to constriction, the individual densities were often spatially separated circumferentially. As the cell envelope constricted, the discontinuous ring formed by FtsZ moved inside the discontinuous ring formed by FtsN. The radial and circumferential separation observed in our images indicates that the majority of FtsZ and FtsN molecules are organized in different macromolecular assemblies, rather than in a large super‐complex. This conclusion was supported by fluorescence recovery after photobleaching measurements, which indicated that the dynamic behavior of the two macromolecular assemblies was also fundamentally different. Taken together, the data indicates that constriction of the cell envelope is brought about by (at least) two spatially separated complexes. | |||||
出版者 | ||||||
出版者 | John Wiley & Sons Ltd | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0950-382X | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1365-2958 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/29193432 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1111/mmi.13888 | |||||
権利 | ||||||
権利情報 | © 2017 John Wiley & Sons Ltd | |||||
権利 | ||||||
権利情報 | This is the peer reviewed version of the following article: Söderström, B. , Chan, H. , Shilling, P. J., Skoglund, U. and Daley, D. O. (2018), Spatial separation of FtsZ and FtsN during cell division. Molecular Microbiology, 107: 387-401. doi:10.1111/mmi.13888, which has been published in final form at https://doi.org/10.1111/mmi.13888. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://onlinelibrary.wiley.com/doi/abs/10.1111/mmi.13888 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |