WEKO3
アイテム
{"_buckets": {"deposit": "4ef3666c-3b98-44cb-b8c4-e244cc10d726"}, "_deposit": {"created_by": 27, "id": "442", "owners": [27], "pid": {"revision_id": 0, "type": "depid", "value": "442"}, "status": "published"}, "_oai": {"id": "oai:oist.repo.nii.ac.jp:00000442", "sets": ["51"]}, "author_link": ["1826", "1829", "1824", "1828", "1818", "1819", "1823", "1817", "1830", "1820", "1827", "1831", "1822", "1821", "1825"], "item_10001_biblio_info_7": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-08-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "8", "bibliographicPageStart": "e0182280", "bibliographicVolumeNumber": "12", "bibliographic_titles": [{}, {"bibliographic_title": "PLoS ONE", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "Author", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Matsuyama, Tomomasa"}], "nameIdentifiers": [{"nameIdentifier": "1817", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yasuike, Motoshige"}], "nameIdentifiers": [{"nameIdentifier": "1818", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujiwara, Atushi"}], "nameIdentifiers": [{"nameIdentifier": "1819", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakamura, Yoji"}], "nameIdentifiers": [{"nameIdentifier": "1820", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takano, Tomokazu"}], "nameIdentifiers": [{"nameIdentifier": "1821", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takeuchi, Takeshi"}], "nameIdentifiers": [{"nameIdentifier": "1822", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Satoh, Noriyuki"}], "nameIdentifiers": [{"nameIdentifier": "1823", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Adachi, Yoshikazu"}], "nameIdentifiers": [{"nameIdentifier": "1824", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsuchihashi, Yasushi"}], "nameIdentifiers": [{"nameIdentifier": "1825", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Aoki, Hideo"}], "nameIdentifiers": [{"nameIdentifier": "1826", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Odawara, Kazushi"}], "nameIdentifiers": [{"nameIdentifier": "1827", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Iwanaga, Shunsuke"}], "nameIdentifiers": [{"nameIdentifier": "1828", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kurita, Jun"}], "nameIdentifiers": [{"nameIdentifier": "1829", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kamaishi, Takashi"}], "nameIdentifiers": [{"nameIdentifier": "1830", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakayasu, Chihaya"}], "nameIdentifiers": [{"nameIdentifier": "1831", "nameIdentifierScheme": "WEKO"}]}]}, "item_10001_description_5": {"attribute_name": "Abstract", "attribute_value_mlt": [{"subitem_description": "Mass mortality that is acompanied by reddish browning of the soft tissues has been occurring in cultured pearl oyster, Pinctada fucata martensii. The disease is called Akoya oyster disease (AOD). Although spreading pattern of the disease and transmission experiments suggest that the disease is infectious, the causative agent has not yet been identified. We used shotgun and 16S rRNA-based metagenomic analysis to identify genes that are present specifically in affected oysters. The genes found only in diseased oysters were mostly bacterial origin, suggesting that the causative agent was a bacterial pathogen. This hypothesis was supported by the inhibition of AOD development in naive oysters injected with the hemolymph of diseased animals followed immediately with penicillin bath-administration. Further analyses of the hemolymph and mantle specifically and universally detected genes of bacteria that belong to phylum Spirochaetes in diseased pearl oysters but not in healthy oysters. By in situ hybridization or immunostaining, a Brachyspira-like bacterium was observed in the smears of hemolymph from affected oysters, but not from healthy oysters. Phylogenetic analysis using 16S rRNA sequences showed that the presumptive causative bacterium was outside of but most closely related to family Brachyspiraceae. We propose \u0027Candidatus Maribrachyspira akoyae\u0027 gen. nov, sp nov., for this bacterium.", "subitem_description_type": "Other"}]}, "item_10001_publisher_8": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_publisher": "PLOS ONE"}]}, "item_10001_relation_13": {"attribute_name": "PubMedNo.", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:pmid/28771537", "subitem_relation_type_select": "PMID"}}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1371/journal.pone.0182280", "subitem_relation_type_select": "DOI"}}]}, "item_10001_relation_17": {"attribute_name": "Related site", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0182280", "subitem_relation_type_select": "URI"}}]}, "item_10001_rights_15": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_rights": " © 2017 Matsuyama et al."}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1932-6203", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_version_type_20": {"attribute_name": "Author\u0027s flag", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-07-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "PLOSone 12(8) e01822802017.pdf", "filesize": [{"value": "20.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "Creative Commons Attribution 4.0 International\nhttps://creativecommons.org/licenses/by/4.0/", "licensetype": "license_free", "mimetype": "application/pdf", "size": 20200000.0, "url": {"label": "PLOSone 12(8) e01822802017", "url": "https://oist.repo.nii.ac.jp/record/442/files/PLOSone 12(8) e01822802017.pdf"}, "version_id": "3a13009d-491d-4fef-92c4-641cb5c1073d"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "A Spirochaete is suggested as the causative agent of Akoya oyster disease by metagenomic analysis", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "A Spirochaete is suggested as the causative agent of Akoya oyster disease by metagenomic analysis", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "27", "path": ["51"], "permalink_uri": "https://oist.repo.nii.ac.jp/records/442", "pubdate": {"attribute_name": "公開日", "attribute_value": "2018-07-24"}, "publish_date": "2018-07-24", "publish_status": "0", "recid": "442", "relation": {}, "relation_version_is_last": true, "title": ["A Spirochaete is suggested as the causative agent of Akoya oyster disease by metagenomic analysis"], "weko_shared_id": 27}
A Spirochaete is suggested as the causative agent of Akoya oyster disease by metagenomic analysis
https://oist.repo.nii.ac.jp/records/442
https://oist.repo.nii.ac.jp/records/4427670c821-3858-494a-91cb-f8052806e48e
名前 / ファイル | ライセンス | アクション |
---|---|---|
PLOSone 12(8) e01822802017 (20.2 MB)
|
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/ |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2018-07-24 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | A Spirochaete is suggested as the causative agent of Akoya oyster disease by metagenomic analysis | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Matsuyama, Tomomasa
× Matsuyama, Tomomasa× Yasuike, Motoshige× Fujiwara, Atushi× Nakamura, Yoji× Takano, Tomokazu× Takeuchi, Takeshi× Satoh, Noriyuki× Adachi, Yoshikazu× Tsuchihashi, Yasushi× Aoki, Hideo× Odawara, Kazushi× Iwanaga, Shunsuke× Kurita, Jun× Kamaishi, Takashi× Nakayasu, Chihaya |
|||||
書誌情報 |
en : PLoS ONE 巻 12, 号 8, p. e0182280, 発行日 2017-08-03 |
|||||
抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Mass mortality that is acompanied by reddish browning of the soft tissues has been occurring in cultured pearl oyster, Pinctada fucata martensii. The disease is called Akoya oyster disease (AOD). Although spreading pattern of the disease and transmission experiments suggest that the disease is infectious, the causative agent has not yet been identified. We used shotgun and 16S rRNA-based metagenomic analysis to identify genes that are present specifically in affected oysters. The genes found only in diseased oysters were mostly bacterial origin, suggesting that the causative agent was a bacterial pathogen. This hypothesis was supported by the inhibition of AOD development in naive oysters injected with the hemolymph of diseased animals followed immediately with penicillin bath-administration. Further analyses of the hemolymph and mantle specifically and universally detected genes of bacteria that belong to phylum Spirochaetes in diseased pearl oysters but not in healthy oysters. By in situ hybridization or immunostaining, a Brachyspira-like bacterium was observed in the smears of hemolymph from affected oysters, but not from healthy oysters. Phylogenetic analysis using 16S rRNA sequences showed that the presumptive causative bacterium was outside of but most closely related to family Brachyspiraceae. We propose 'Candidatus Maribrachyspira akoyae' gen. nov, sp nov., for this bacterium. | |||||
出版者 | ||||||
出版者 | PLOS ONE | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1932-6203 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/28771537 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1371/journal.pone.0182280 | |||||
権利 | ||||||
権利情報 | © 2017 Matsuyama et al. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0182280 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |