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Detection of antibodies against SARS-CoV-2 spike protein by gold nanospikes in an opto-microfluidic chip
https://oist.repo.nii.ac.jp/records/1902
https://oist.repo.nii.ac.jp/records/190257c904ae-ec65-43d0-8ae0-e43706f6c6f1
名前 / ファイル | ライセンス | アクション |
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Accepted (6.5 MB)
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International(https://creativecommons.org/licenses/by-nc-nd/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-01-20 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Detection of antibodies against SARS-CoV-2 spike protein by gold nanospikes in an opto-microfluidic chip | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | SARS-CoV-2 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | COVID-19 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Antibody | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | LSPR | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Microfluidics | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Gold electrodeposition | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Funari, Riccardo
× Funari, Riccardo× Chu, Kang-Yu× Shen, Amy Q. |
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書誌情報 |
en : Biosensors and Bioelectronics 巻 169, p. 112578, 発行日 2020-09-03 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The ongoing global pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to active research in its associated diagnostics and medical treatments. While quantitative reverse transcription polymerase chain reaction (qRT-PCR) is the most reliable method to detect viral genes of SARS-CoV-2, serological tests for specific antiviral antibodies are also important as they identify false negative qRT-PCR responses, track how effectively the patient's immune system is fighting the infection, and are potentially helpful for plasma transfusion therapies. In this work, based on the principle of localized surface plasmon resonance (LSPR), we develop an opto-microfluidic sensing platform with gold nanospikes, fabricated by electrodeposition, to detect the presence and amount of antibodies specific to the SARS-CoV-2 spike protein in 1 mu L of human plasma diluted in 1 mL of buffer solution, within similar to 30 min. The target antibody concentration can be correlated with the LSPR wavelength peak shift of gold nanospikes caused by the local refractive index change due to the antigen-antibody binding. This label-free microfluidic platform achieves a limit of detection of similar to 0.08 ng/mL (similar to 0.5 pM), falling under the clinical relevant concentration range. We demonstrate that our opto-microfluidic platform offers a promising point-of-care testing tool to complement standard serological assays and make SARS-CoV-2 quantitative diagnostics easier, cheaper, and faster. | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0956-5663 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/32911317 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.bios.2020.112578 | |||||
権利 | ||||||
権利情報 | This article/chapter was published in Biosensors and Bioelectronics, Volume 169, Riccardo Funari, Kang-Yu Chu, Amy Q. Shen, Detection of antibodies against SARS-CoV-2 spike protein by gold nanospikes in an opto-microfluidic chip, Page 112578, © Elsevier 2020. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.sciencedirect.com/science/article/pii/S0956566320305686 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |