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An OregonGreen488-labelled d-amino acid for visualizing peptidoglycan by super-resolution STED nanoscopy
https://oist.repo.nii.ac.jp/records/1931
https://oist.repo.nii.ac.jp/records/19310db4772b-96ef-4590-8da5-d639b4d0142a
名前 / ファイル | ライセンス | アクション |
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1129_micro000996 (1.3 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-02-12 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | An OregonGreen488-labelled d-amino acid for visualizing peptidoglycan by super-resolution STED nanoscopy | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | FDAA | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | FLDA | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | OGDA | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | OregonGreen488 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | peptidoglycan | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | transpeptidase | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Söderström, Bill
× Söderström, Bill× Ruda, Alessandro× Widmalm, Göran× Daley, Daniel O. |
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書誌情報 |
en : Microbiology 巻 166, 号 12, p. 1129-1135, 発行日 2020-11-25 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Fluorescent d-amino acids (FDAAs) are molecular probes that are widely used for labelling the peptidoglycan layer of bacteria. When added to growing cells they are incorporated into the stem peptide by a transpeptidase reaction, allowing the timing and localization of peptidoglycan synthesis to be determined by fluorescence microscopy. Herein we describe the chemical synthesis of an OregonGreen488-labelled FDAA (OGDA). We also demonstrate that OGDA can be efficiently incorporated into the PG of Gram-positive and some Gram-negative bacteria, and imaged by super-resolution stimulated emission depletion (STED) nanoscopy at a resolution well below 100 nm. | |||||
出版者 | ||||||
出版者 | Microbiology Society | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1350-0872 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1465-2080 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/33237852 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1099/mic.0.000996 | |||||
権利 | ||||||
権利情報 | © 2020 The Author(s). | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000996#html | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |