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Green fluorescent cAMP indicator of high speed and specificity suitable for neuronal live-cell imaging
https://oist.repo.nii.ac.jp/records/2896
https://oist.repo.nii.ac.jp/records/28964bfcbaa4-c95f-4808-8e71-697d5e8d29a0
名前 / ファイル | ライセンス | アクション |
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pnas.2122618119 (1.3 MB)
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (https://creativecommons.org/licenses/by-nc-nd/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2023-01-25 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Green fluorescent cAMP indicator of high speed and specificity suitable for neuronal live-cell imaging | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cAMP | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | neuron | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | live cell imaging | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | indicator | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Kawata, Seiko
× Kawata, Seiko× Mukai, Yuki× Nishimura, Yumi× Takahashi, Tomoyuki× Saitoh, Naoto |
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書誌情報 |
en : Proceedings of the National Academy of Sciences 巻 119, 号 28, p. e2122618119, 発行日 2022-07-06 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Cyclic adenosine monophosphate (cAMP) is a canonical intracellular messenger playing diverse roles in cell functions. In neurons, cAMP promotes axonal growth during early development, and mediates sensory transduction and synaptic plasticity after maturation. The molecular cascades of cAMP are well documented, but its spatiotemporal profiles associated with neuronal functions remain hidden. Hence, we developed a genetically encoded cAMP indicator based on a bacterial cAMP-binding protein. This indicator “gCarvi” monitors [cAMP]i at 0.2 to 20 µM with a subsecond time resolution and a high specificity over cyclic guanosine monophosphate (cGMP). gCarvi can be converted to a ratiometric probe for [cAMP]i quantification and its expression can be specifically targeted to various subcellular compartments. Monomeric gCarvi also enables simultaneous multisignal monitoring in combination with other indicators. As a proof of concept, simultaneous cAMP/Ca2+ imaging in hippocampal neurons revealed a tight linkage of cAMP to Ca2+ signals. In cerebellar presynaptic boutons, forskolin induced nonuniform cAMP elevations among boutons, which positively correlated with subsequent increases in the size of the recycling pool of synaptic vesicles assayed using FM dye. Thus, the cAMP domain in presynaptic boutons is an important determinant of the synaptic strength. | |||||
出版者 | ||||||
出版者 | PNAS | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0027-8424 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1091-6490 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/35867738 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1073/pnas.2122618119 | |||||
権利 | ||||||
権利情報 | © 2022 The Authors. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.pnas.org/doi/10.1073/pnas.2122618119 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |