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Reversible Gene Regulation in Mammalian Cells Using Riboswitch-Engineered Vesicular Stomatitis Virus Vector
https://oist.repo.nii.ac.jp/records/1236
https://oist.repo.nii.ac.jp/records/12361e3f010b-6b45-40e5-9cc9-f264ae4dcea2
名前 / ファイル | ライセンス | アクション |
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Takahashi-2019-Reversible Gene Regulation in M (1.6 MB)
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ACS AuthorChoice
(https://pubs.acs.org/page/policy/authorchoice_termsofuse.html) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-03-06 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Reversible Gene Regulation in Mammalian Cells Using Riboswitch-Engineered Vesicular Stomatitis Virus Vector | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Takahashi, Kei
× Takahashi, Kei× Yokobayashi, Yohei |
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書誌情報 |
en : ACS Synthetic Biology 巻 8, 号 9, p. 1976-1982, 発行日 2019-08-15 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Synthetic riboswitches based on small molecule-responsive self-cleaving ribozymes (aptazymes) embedded in the untranslated regions (UTRs) allow chemical control of gene expression in mammalian cells. In this work, we used a guanine-responsive aptazyme to control transgene expression from a replication-incompetent vesicular stomatitis virus (VSV) vector. VSV is a nonsegmented, negative-sense, cytoplasmic RNA virus that replicates without DNA intermediates, and its applications for vaccines and oncolytic viral therapy are being explored. By inserting the guanine-activated ribozyme in the 3′ UTRs of viral genes and transgenes, GFP expression from the VSV vector in mammalian cells was repressed by as much as 26.8-fold in the presence of guanine. Furthermore, we demonstrated reversible regulation of a transgene (secreted NanoLuc) by adding and withdrawing guanine from the medium over the course of 12 days. In summary, our riboswitch-controlled VSV vector allows robust, long-term, and reversible regulation of gene expression in mammalian cells without the risk of undesirable genomic integration. | |||||
出版者 | ||||||
出版者 | American Chemical Society | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2161-5063 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/31415142 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1021/acssynbio.9b00177 | |||||
権利 | ||||||
権利情報 | © 2019 American Chemical Society. | |||||
情報源 | ||||||
関連名称 | https://pubs.acs.org/page/policy/authorchoice_termsofuse.html | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://pubs.acs.org/doi/abs/10.1021/acssynbio.9b00177 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |