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psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation
https://oist.repo.nii.ac.jp/records/2073
https://oist.repo.nii.ac.jp/records/2073769b7fdb-a0dc-46fa-9ca5-4d30f8880add
名前 / ファイル | ライセンス | アクション |
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Strittmatter-2021-psiCLIP reveals dynamic RNA (3.3 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-05-14 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Strittmatter, Lisa M.
× Strittmatter, Lisa M.× Capitanchik, Charlotte× Newman, Andrew J.× Hallegger, Martina× Norman, Christine M.× Fica, Sebastian M.× Oubridge, Chris× Luscombe, Nicholas M.× Ule, Jernej× Nagai, Kiyoshi |
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書誌情報 |
en : Nature Communications 巻 12, 号 1, p. 1488, 発行日 2021-03-05 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | RNA helicases remodel the spliceosome to enable pre-mRNA splicing, but their binding and mechanism of action remain poorly understood. To define helicase-RNA contacts in specific spliceosomal states, we develop purified spliceosome iCLIP (psiCLIP), which reveals dynamic helicase-RNA contacts during splicing catalysis. The helicase Prp16 binds along the entire available single-stranded RNA region between the branchpoint and 3'-splice site, while Prp22 binds diffusely downstream of the branchpoint before exon ligation, but then switches to more narrow binding in the downstream exon after exon ligation, arguing against a mechanism of processive translocation. Depletion of the exon-ligation factor Prp18 destabilizes Prp22 binding to the pre-mRNA, suggesting that proofreading by Prp22 may sense the stability of the spliceosome during exon ligation. Thus, psiCLIP complements structural studies by providing key insights into the binding and proofreading activity of spliceosomal RNA helicases. | |||||
出版者 | ||||||
出版者 | Nature Portfolio | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2041-1723 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/33674615 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1038/s41467-021-21745-9 | |||||
権利 | ||||||
権利情報 | © 2021 The Author(s). | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.nature.com/articles/s41467-021-21745-9 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |