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Large Scale Mutational and Kinetic Analysis of a Self-Hydrolyzing Deoxyribozyme
https://oist.repo.nii.ac.jp/records/366
https://oist.repo.nii.ac.jp/records/366053995fb-f8dc-4f23-9b17-3f4251de1b32
名前 / ファイル | ライセンス | アクション |
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acschembio.7b00621 (1.7 MB)
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ACS AuthorChoice
(https://pubs.acs.org/page/policy/authorchoice_termsofuse.html) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-04-12 | |||||
タイトル | ||||||
タイトル | Large Scale Mutational and Kinetic Analysis of a Self-Hydrolyzing Deoxyribozyme | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Dhamodharan, V.
× Dhamodharan, V.× Kobori, Shungo× Yokobayashi, Yohei |
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書誌情報 |
en : ACS Chemical Biology 巻 12, 号 12, p. 2940-2945, 発行日 2017-10-23 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Deoxyribozymes are catalytic DNA sequences whose atomic structures are generally difficult to elucidate. Mutational analysis remains a principal approach for understanding and engineering deoxyribozymes with diverse catalytic activities. However, laborious preparation and biochemical characterization of individual sequences severely limit the number of mutants that can be studied biochemically. Here, we applied deep sequencing to directly measure the activities of self-hydrolyzing deoxyribozyme sequences in high throughput. First, all single and double mutants within the 15-base catalytic core of the deoxyribozyme I-R3 were assayed to unambiguously determine the tolerated and untolerated mutations at each position. Subsequently, 4096 deoxyribozyme variants with tolerated base substitutions at seven positions were kinetically assayed in parallel. We identified 533 active mutants whose first-order rate constants and activation energies were determined. The results indicate an isolated and narrow peak in the deoxyribozyme sequence space and provide a quantitative view of the effects of multiple mutations on the deoxyribozyme activity for the first time. | |||||
出版者 | ||||||
出版者 | American Chemical Society | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1554-8929 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1554-8937 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/29058875 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1021/acschembio.7b00621 | |||||
権利 | ||||||
権利情報 | © 2017 American Chemical Society. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://pubs.acs.org/doi/10.1021/acschembio.7b00621 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |