WEKO3
アイテム
Thyroid hormone receptor beta mutations alter photoreceptor development and function in Danio rerio (zebrafish)
https://oist.repo.nii.ac.jp/records/1657
https://oist.repo.nii.ac.jp/records/165770638997-880b-4ff0-a559-7ea52b4116e1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
journal.pgen.1008869 (7.3 MB)
|
Creative Commons CC0 1.0 Universal(https://creativecommons.org/publicdomain/zero/1.0/)
|
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2020-07-31 | |||||||||||||||||||
| タイトル | ||||||||||||||||||||
| タイトル | Thyroid hormone receptor beta mutations alter photoreceptor development and function in Danio rerio (zebrafish) | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 言語 | ||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||
| 著者（英） |
Deveau, Ciana
× Deveau, Ciana
× Jiao, Xiaodong
× Suzuki, Sachihiro C.
× Krishnakumar, Asha
× Yoshimatsu, Takeshi
× Hejtmancik, J Fielding
× Nelson, Ralph F.
|
|||||||||||||||||||
| 書誌情報 |
en : PLOS Genetics 巻 16, 号 6, p. e1008869, 発行日 2020-06-22 |
|||||||||||||||||||
| 抄録 | ||||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||||
| 内容記述 | We investigate mutations in trbeta2, a splice variant of thrb, identifying changes in function, structure, and behavior in larval and adult zebrafish retinas. Two N-terminus CRISPR mutants were identified. The first is a 6BP+1 insertion deletion frameshift resulting in a truncated protein. The second is a 3BP in frame deletion with intact binding domains. ERG recordings of isolated cone signals showed that the 6BP+1 mutants did not respond to red wavelengths of light while the 3BP mutants did respond. 6BP+1 mutants lacked optomotor and optokinetic responses to red/black and green/black contrasts. Both larval and adult 6BP+1 mutants exhibit a loss of red-cone contribution to the ERG and an increase in UV-cone contribution. Transgenic reporters show loss of cone trbeta2 activation in the 6BP+1 mutant but increase in the density of cones with active blue, green, and UV opsin genes. Antibody reactivity for red-cone LWS1 and LWS2 opsin was absent in the 6BP+1 mutant, as was reactivity for arrestin3a. Our results confirm a critical role for trbeta2 in long-wavelength cone development. | |||||||||||||||||||
| 出版者 | ||||||||||||||||||||
| 出版者 | Public Library of Science | |||||||||||||||||||
| ISSN | ||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||
| 収録物識別子 | 1553-7404 | |||||||||||||||||||
| ISSN | ||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||
| 収録物識別子 | 1553-7390 | |||||||||||||||||||
| PubMed番号 | ||||||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||||||
| 識別子タイプ | PMID | |||||||||||||||||||
| 関連識別子 | info:pmid/32569302 | |||||||||||||||||||
| DOI | ||||||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||
| 関連識別子 | info:doi/10.1371/journal.pgen.1008869 | |||||||||||||||||||
| 情報源 | ||||||||||||||||||||
| 関連名称 | https://creativecommons.org/publicdomain/zero/1.0/ | |||||||||||||||||||
| 関連サイト | ||||||||||||||||||||
| 識別子タイプ | URI | |||||||||||||||||||
| 関連識別子 | https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1008869 | |||||||||||||||||||
| 著者版フラグ | ||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||
