WEKO3
アイテム
{"_buckets": {"deposit": "019dea0b-8ff2-4d1d-9081-9b35539f7d25"}, "_deposit": {"created_by": 29, "id": "1657", "owners": [29], "pid": {"revision_id": 0, "type": "depid", "value": "1657"}, "status": "published"}, "_oai": {"id": "oai:oist.repo.nii.ac.jp:00001657", "sets": ["218"]}, "author_link": ["9945", "9943", "9941", "9940", "9942", "9939", "9944"], "item_10001_biblio_info_7": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-06-22", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageStart": "e1008869", "bibliographicVolumeNumber": "16", "bibliographic_titles": [{}, {"bibliographic_title": "PLOS Genetics", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "Author", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Deveau, Ciana"}], "nameIdentifiers": [{"nameIdentifier": "9939", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Jiao, Xiaodong"}], "nameIdentifiers": [{"nameIdentifier": "9940", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Sachihiro C."}], "nameIdentifiers": [{"nameIdentifier": "9941", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Krishnakumar, Asha"}], "nameIdentifiers": [{"nameIdentifier": "9942", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshimatsu, Takeshi"}], "nameIdentifiers": [{"nameIdentifier": "9943", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hejtmancik, J Fielding"}], "nameIdentifiers": [{"nameIdentifier": "9944", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nelson, Ralph F."}], "nameIdentifiers": [{"nameIdentifier": "9945", "nameIdentifierScheme": "WEKO"}]}]}, "item_10001_description_5": {"attribute_name": "Abstract", "attribute_value_mlt": [{"subitem_description": "We investigate mutations in trbeta2, a splice variant of thrb, identifying changes in function, structure, and behavior in larval and adult zebrafish retinas. Two N-terminus CRISPR mutants were identified. The first is a 6BP+1 insertion deletion frameshift resulting in a truncated protein. The second is a 3BP in frame deletion with intact binding domains. ERG recordings of isolated cone signals showed that the 6BP+1 mutants did not respond to red wavelengths of light while the 3BP mutants did respond. 6BP+1 mutants lacked optomotor and optokinetic responses to red/black and green/black contrasts. Both larval and adult 6BP+1 mutants exhibit a loss of red-cone contribution to the ERG and an increase in UV-cone contribution. Transgenic reporters show loss of cone trbeta2 activation in the 6BP+1 mutant but increase in the density of cones with active blue, green, and UV opsin genes. Antibody reactivity for red-cone LWS1 and LWS2 opsin was absent in the 6BP+1 mutant, as was reactivity for arrestin3a. Our results confirm a critical role for trbeta2 in long-wavelength cone development.", "subitem_description_type": "Other"}]}, "item_10001_publisher_8": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_publisher": "Public Library of Science"}]}, "item_10001_relation_13": {"attribute_name": "PubMedNo.", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:pmid/32569302", "subitem_relation_type_select": "PMID"}}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1371/journal.pgen.1008869", "subitem_relation_type_select": "DOI"}}]}, "item_10001_relation_16": {"attribute_name": "情報源", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "https://creativecommons.org/publicdomain/zero/1.0/"}]}]}, "item_10001_relation_17": {"attribute_name": "Related site", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1008869", "subitem_relation_type_select": "URI"}}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1553-7404", "subitem_source_identifier_type": "ISSN"}, {"subitem_source_identifier": "1553-7390", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_version_type_20": {"attribute_name": "Author\u0027s flag", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-07-31"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "journal.pgen.1008869.pdf", "filesize": [{"value": "7.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "Creative Commons CC0 1.0 Universal(https://creativecommons.org/publicdomain/zero/1.0/)", "licensetype": "license_free", "mimetype": "application/pdf", "size": 7300000.0, "url": {"label": "journal.pgen.1008869", "url": "https://oist.repo.nii.ac.jp/record/1657/files/journal.pgen.1008869.pdf"}, "version_id": "36264d01-59b9-43cb-9249-28e89ba1e2a2"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Thyroid hormone receptor beta mutations alter photoreceptor development and function in Danio rerio (zebrafish)", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Thyroid hormone receptor beta mutations alter photoreceptor development and function in Danio rerio (zebrafish)", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "29", "path": ["218"], "permalink_uri": "https://oist.repo.nii.ac.jp/records/1657", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-07-31"}, "publish_date": "2020-07-31", "publish_status": "0", "recid": "1657", "relation": {}, "relation_version_is_last": true, "title": ["Thyroid hormone receptor beta mutations alter photoreceptor development and function in Danio rerio (zebrafish)"], "weko_shared_id": 29}
Thyroid hormone receptor beta mutations alter photoreceptor development and function in Danio rerio (zebrafish)
https://oist.repo.nii.ac.jp/records/1657
https://oist.repo.nii.ac.jp/records/165770638997-880b-4ff0-a559-7ea52b4116e1
名前 / ファイル | ライセンス | アクション |
---|---|---|
journal.pgen.1008869 (7.3 MB)
|
Creative Commons CC0 1.0 Universal(https://creativecommons.org/publicdomain/zero/1.0/)
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2020-07-31 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Thyroid hormone receptor beta mutations alter photoreceptor development and function in Danio rerio (zebrafish) | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Deveau, Ciana
× Deveau, Ciana× Jiao, Xiaodong× Suzuki, Sachihiro C.× Krishnakumar, Asha× Yoshimatsu, Takeshi× Hejtmancik, J Fielding× Nelson, Ralph F. |
|||||
書誌情報 |
en : PLOS Genetics 巻 16, 号 6, p. e1008869, 発行日 2020-06-22 |
|||||
抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | We investigate mutations in trbeta2, a splice variant of thrb, identifying changes in function, structure, and behavior in larval and adult zebrafish retinas. Two N-terminus CRISPR mutants were identified. The first is a 6BP+1 insertion deletion frameshift resulting in a truncated protein. The second is a 3BP in frame deletion with intact binding domains. ERG recordings of isolated cone signals showed that the 6BP+1 mutants did not respond to red wavelengths of light while the 3BP mutants did respond. 6BP+1 mutants lacked optomotor and optokinetic responses to red/black and green/black contrasts. Both larval and adult 6BP+1 mutants exhibit a loss of red-cone contribution to the ERG and an increase in UV-cone contribution. Transgenic reporters show loss of cone trbeta2 activation in the 6BP+1 mutant but increase in the density of cones with active blue, green, and UV opsin genes. Antibody reactivity for red-cone LWS1 and LWS2 opsin was absent in the 6BP+1 mutant, as was reactivity for arrestin3a. Our results confirm a critical role for trbeta2 in long-wavelength cone development. | |||||
出版者 | ||||||
出版者 | Public Library of Science | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1553-7404 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1553-7390 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/32569302 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1371/journal.pgen.1008869 | |||||
情報源 | ||||||
関連名称 | https://creativecommons.org/publicdomain/zero/1.0/ | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1008869 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |