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Desaturase specificity is controlled by the physicochemical properties of a single amino acid residue in the substrate binding tunnel
https://oist.repo.nii.ac.jp/records/1924
https://oist.repo.nii.ac.jp/records/19240d10b0af-75f2-4396-80f5-7a8d560b741b
名前 / ファイル | ライセンス | アクション |
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Bucek-2020-Desaturase specificity is controlle (1.6 MB)
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International(https://creativecommons.org/licenses/by-nc-nd/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-02-09 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Desaturase specificity is controlled by the physicochemical properties of a single amino acid residue in the substrate binding tunnel | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Acyl-CoA desaturase | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Manduca sexta | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Bombyx mori | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Enzymatic specificity | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Protein engineering | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Molecular dynamics simulations | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Buček, Aleš
× Buček, Aleš× Vazdar, Mario× Tupec, Michal× Svatoš, Aleš× Pichová, Iva |
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書誌情報 |
en : Computational and Structural Biotechnology Journal 巻 18, p. 1202-1209, 発行日 2020-05-14 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Membrane fatty acyl desaturases (mFAD) are ubiquitous enzymes in eukaryotes. They introduce double bonds into fatty acids (FAs), producing structurally diverse unsaturated FAs which serve as membrane lipid components or precursors of signaling molecules. The mechanisms controlling enzymatic specificity and selectivity of desaturation are, however, poorly understood. We found that the physicochemical properties, particularly side chain volume, of a single amino acid (aa) residue in insect mFADs (Lepidoptera: Bombyx mori and Manduca sexta) control the desaturation products. Molecular dynamics simulations of systems comprising wild-type or mutant mFADs with fatty acyl-CoA substrates revealed that the single aa substitution likely directs the outcome of the desaturation reaction by modulating the distance between substrate fatty acyl carbon atoms and active center metal ions. These findings, as well as our methodology combining mFAD mutational screening with molecular dynamics simulations, will facilitate prediction of desaturation products and facilitate engineering of mFADs for biotechnological applications. | |||||
出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2001-0370 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/32542106 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.csbj.2020.05.011 | |||||
権利 | ||||||
権利情報 | © 2020 The Author(s). | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.sciencedirect.com/science/article/pii/S2001037020302749 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |