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An autoregulation loop in fust-1 for circular RNA regulation in Caenorhabditis elegans
https://oist.repo.nii.ac.jp/records/2384
https://oist.repo.nii.ac.jp/records/238413cec98f-11de-4dc7-964c-a8dd206f5951
名前 / ファイル | ライセンス | アクション |
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GENETICS-2021-304543R1_Merged_PDF (6.7 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-11-24 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | An autoregulation loop in fust-1 for circular RNA regulation in Caenorhabditis elegans | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | circular RNA | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | FUS | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | RNA-binding protein | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | autoregulation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | back splicing | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | alternative splicing | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Cao, Dong
× Cao, Dong |
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書誌情報 |
en : Genetics 巻 219, 号 3, p. iyab145, 発行日 2021-09-07 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Many circular RNAs (circRNAs) are differentially expressed in different tissues or cell types, suggestive of specific factors that regulate their biogenesis. Here, taking advantage of available mutation strains of RNA-binding proteins (RBPs) in Caenorhabditis elegans, I performed a screening of circRNA regulation in 13 conserved RBPs. Among them, loss of FUST-1, the homolog of Fused in Sarcoma (FUS), caused downregulation of multiple circRNAs. By rescue experiments, I confirmed FUST-1 as a circRNA regulator. Through RNA sequencing using circRNA-enriched samples, circRNAs targets regulated by FUST-1 were identified globally, with hundreds of them significantly altered. Furthermore, I showed that FUST-1 regulates circRNA formation with only small to little effect on the cognate linear mRNAs. When recognizing circRNA pre-mRNAs, FUST-1 can affect both exon-skipping and circRNA in the same genes. Moreover, I identified an autoregulation loop in fust-1, where FUST-1, isoform a (FUST-1A) promotes the skipping of exon 5 of its own pre-mRNA, which produces FUST-1, isoform b (FUST-1B) with different N-terminal sequences. FUST-1A is the functional isoform in circRNA regulation. Although FUST-1B has the same functional domains as FUST-1A, it cannot regulate either exon-skipping or circRNA formation. This study provided an in vivo investigation of circRNA regulation, which will be helpful to understand the mechanisms that govern circRNA formation. | |||||
出版者 | ||||||
出版者 | Oxford University Press on behalf of Genetics Society of America. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1943-2631 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0016-6731 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/34740247 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1093/genetics/iyab145 | |||||
権利 | ||||||
権利情報 | This is a pre-copyedited, author-produced version of an article accepted for publication in Genetics following peer review. The version of record, Dong Cao, An autoregulation loop in fust-1 for circular RNA regulation in Caenorhabditis elegans, Genetics, Volume 219, Issue 3, September 2021, iyab145, is available online at: https://doi.org/10.1093/genetics/iyab145. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://academic.oup.com/genetics/article/219/3/iyab145/6365942 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |