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Riboswitch Signal Amplification by Controlling Plasmid Copy Number
https://oist.repo.nii.ac.jp/records/1068
https://oist.repo.nii.ac.jp/records/10682fe85f7c-1d1a-434a-83a2-79e11a387c53
名前 / ファイル | ライセンス | アクション |
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acssynbio.8b00454 (1.5 MB)
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ACS AuthorChoice
(https://pubs.acs.org/page/policy/authorchoice_termsofuse.html) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-08-28 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Riboswitch Signal Amplification by Controlling Plasmid Copy Number | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Dwidar, Mohammed
× Dwidar, Mohammed× Yokobayashi, Yohei |
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書誌情報 |
en : ACS Synthetic Biology 巻 8, 号 2, p. 245-250, 発行日 2019-01-25 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Riboswitches are cis-acting RNA devices in mRNAs that control gene expression in response to chemical inputs. As RNA aptamers that recognize diverse classes of molecules can be isolated by in vitro selection, synthetic riboswitches hold promise for various applications in synthetic biology. One of the major drawbacks of riboswitches, however, is their limited dynamic range. A high level of gene expression in the OFF state (leakage) is also a common problem. To address these challenges, we designed and constructed a dual-riboswitch plasmid in which two genes are controlled by theophylline-activated riboswitches. One riboswitch controls the gene of interest, and another riboswitch controls RepL, a phage-derived replication protein that regulates the plasmid copy number. This single-plasmid system afforded an ON/OFF ratio as high as 3900. Furthermore, we used the system to control CRISPR interference (CRISPRi) targeting endogenous genes, and successfully observed expected phenotypic changes in Escherichia coli. | |||||
出版者 | ||||||
出版者 | American Chemical Society | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2161-5063 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/30682247 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1021/acssynbio.8b00454 | |||||
権利 | ||||||
権利情報 | © 2019 American Chemical Society | |||||
情報源 | ||||||
関連名称 | https://pubs.acs.org/page/policy/authorchoice_termsofuse.html | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://pubs.acs.org/doi/10.1021/acssynbio.8b00454# | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |