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Quantifying the efficiency and biases of forest Saccharomyces sampling strategies
https://oist.repo.nii.ac.jp/records/1114
https://oist.repo.nii.ac.jp/records/111498e2cf16-cb94-4945-a41a-fc27aa5b8154
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Boynton_et_al-2019-Yeast (731.6 kB)
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Creative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-10-15 | |||||
| タイトル | ||||||
| タイトル | Quantifying the efficiency and biases of forest Saccharomyces sampling strategies | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者（英） |
Boynton, Primrose J.
× Boynton, Primrose J.× Kowallik, Vienna× Landermann, Doreen× Stukenbrock, Eva H. |
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| 書誌情報 |
en : Yeast 号 2019, p. 1-12, 発行日 2019-07-26 |
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| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Saccharomyces yeasts are emerging as model organisms for ecology and evolution, and researchers need environmental Saccharomyces isolates to test ecological and evolutionary hypotheses. However, methods for isolating Saccharomyces from nature have not been standardized, and isolation methods may influence the genotypes and phenotypes of studied strains. We compared the effectiveness and potential biases of an established enrichment culturing method against a newly developed direct plating method for isolating forest floor Saccharomyces spp. In a European forest, enrichment culturing was both less successful at isolating Saccharomyces paradoxus per sample collected and less labour intensive per isolated S. paradoxus colony than direct isolation. The two methods sampled similar S. paradoxus diversity: The number of unique genotypes sampled (i.e., genotypic diversity) per S. paradoxus isolate and average growth rates of S. paradoxus isolates did not differ between the two methods, and growth rate variances (i.e., phenotypic diversity) only differed in one of three tested environments. However, enrichment culturing did detect rare Saccharomyces cerevisiae in the forest habitat and also found two S. paradoxus isolates with outlier phenotypes. Our results validate the historically common method of using enrichment culturing to isolate representative collections of environmental Saccharomyces. We recommend that researchers choose a Saccharomyces sampling method based on resources available for sampling and isolate screening. Researchers interested in discovering new Saccharomyces phenotypes or rare Saccharomyces species from natural environments may also have more success using enrichment culturing. We include step-by-step sampling protocols in the supplemental materials. | |||||
| 出版者 | ||||||
| 出版者 | John Wiley & Sons Ltd | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0749-503X | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1097-0061 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | info:pmid/31348543 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1002/yea.3435 | |||||
| 権利 | ||||||
| 権利情報 | © 2019 The Author(s). | |||||
| 情報源 | ||||||
| 関連名称 | https://creativecommons.org/licenses/by/4.0/ | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://onlinelibrary.wiley.com/doi/10.1002/yea.3435 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
