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Rebuilding a realistic corticostriatal “social network” from dissociated cells
https://oist.repo.nii.ac.jp/records/1208
https://oist.repo.nii.ac.jp/records/120853e815dd-92a8-4957-b7b0-6e5367ee76ba
名前 / ファイル | ライセンス | アクション |
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Garcia-Munoz-2015-Rebuilding a realistic corti (2.5 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-02-27 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Rebuilding a realistic corticostriatal “social network” from dissociated cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Garcia-Munoz, Marianela
× Garcia-Munoz, Marianela× Taillefer, Eddy× Pnini, Reuven× Vickers, Catherine× Miller, Jonathan× Arbuthnott, Gordon W. |
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書誌情報 |
en : Frontiers in Systems Neuroscience 巻 9, p. 63, 発行日 2015-04-20 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Many of the methods available for the study of cortical influences on striatal neurons have serious problems. In vivo the connectivity is so complex that the study of input from an individual cortical neuron to a single striatal cell is nearly impossible. Mixed corticostriatal cultures develop many connections from striatal cells to cortical cells, in striking contrast to the fact that only connections from cortical cells to striatal cells are present in vivo. Furthermore, interneuron populations are over-represented in organotypic cultures. For these reasons, we have developed a method for growing cortical and striatal neurons in separated compartments that allows cortical neurons to innervate striatal cells in culture. The method works equally well for acutely dissociated or cryopreserved neurons and allows a number of manipulations that are not otherwise possible. Either cortical or striatal compartments can be transfected with channel rhodopsins. The activity of both areas can be recorded in multielectrode arrays or individual patch recordings from pairs of cells. Finally, corticostriatal connections can be severed acutely. This procedure enables determination of the importance of corticostriatal interaction in the resting pattern of activity. These cultures also facilitate development of sensitive analytical network methods to track connectivity. | |||||
出版者 | ||||||
出版者 | Frontiers Media | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1662-5137 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/25941477 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.3389/fnsys.2015.00063 | |||||
権利 | ||||||
権利情報 | © 2015 Garcia-Munoz, Taillefer, Pnini, Vickers, Miller and Arbuthnott. | |||||
情報源 | ||||||
関連名称 | https://creativecommons.org/licenses/by/4.0/ | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.frontiersin.org/articles/10.3389/fnsys.2015.00063/full | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |