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Use of Cap Analysis Gene Expression to detect human papillomavirus promoter activity patterns at different disease stages
https://oist.repo.nii.ac.jp/records/1945
https://oist.repo.nii.ac.jp/records/1945a555bfeb-b615-4642-a126-3ccdb91e9565
名前 / ファイル | ライセンス | アクション |
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Taguchi-2020-Use of Cap Analysis Gene Expressi (1.9 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-02-24 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Use of Cap Analysis Gene Expression to detect human papillomavirus promoter activity patterns at different disease stages | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Taguchi, Ayumi
× Taguchi, Ayumi× Nagasaka, Kazunori× Plessy, Charles× Nakamura, Hiroe× Kawata, Yoshiko× Kato, Sachi× Hashimoto, Kosuke× Nagamatsu, Takeshi× Oda, Katsutoshi× Kukimoto, Iwao× Kawana, Kei× Carninci, Piero× Osuga, Yutaka× Fujii, Tomoyuki |
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書誌情報 |
en : Scientific Reports 巻 10, 号 1, p. 17991, 発行日 2020-10-22 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Transcription of human papillomavirus (HPV) genes proceeds unidirectionally from multiple promoters. Direct profiling of transcription start sites (TSSs) by Cap Analysis Gene Expression (CAGE) is a powerful strategy for examining individual HPV promoter activity. The objective of this study was to evaluate alterations of viral promoter activity during infection using CAGE technology. We used CAGE-based sequencing of 46 primary cervical samples, and quantitatively evaluated TSS patterns in the HPV transcriptome at a single-nucleotide resolution. TSS patterns were classified into two types: early promoter-dominant type (Type A) and late promoter-dominant type (Type B). The Type B pattern was more frequently found in CIN1 and CIN2 lesions than in CIN3 and cancer samples. We detected transcriptomes from multiple HPV types in five samples. Interestingly, in each sample, the TSS patterns of both HPV types were the same. The viral gene expression pattern was determined by the differentiation status of the epithelial cells, regardless of HPV type. We performed unbiased analyses of TSSs across the HPV genome in clinical samples. Visualising TSS pattern dynamics, including TSS shifts, provides new insights into how HPV infection status relates to disease state. | |||||
出版者 | ||||||
出版者 | Nature Research | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2045-2322 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/33093512 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1038/s41598-020-75133-2 | |||||
権利 | ||||||
権利情報 | © 2020 The Author(s). | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.nature.com/articles/s41598-020-75133-2 |