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A toolkit for studying Varroa genomics and transcriptomics: preservation, extraction, and sequencing library preparation
https://oist.repo.nii.ac.jp/records/1954
https://oist.repo.nii.ac.jp/records/1954d43711a5-6606-4944-a904-013159d6c1b8
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Hasegawa-2021-A toolkit for studying Varroa ge (2.4 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-03-02 | |||||
| タイトル | ||||||
| タイトル | A toolkit for studying Varroa genomics and transcriptomics: preservation, extraction, and sequencing library preparation | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者（英） |
Hasegawa, Nonno
× Hasegawa, Nonno× Techer, Maeva× Mikheyev, Alexander S. |
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| 書誌情報 |
en : BMC Genomics 巻 22, 号 1, p. 54, 発行日 2021-01-14 |
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| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Background The honey bee parasite, Varroa destructor, is a leading cause of honey bee population declines. In addition to being an obligate ectoparasitic mite, Varroa carries several viruses that infect honey bees and act as the proximal cause of colony collapses. Nevertheless, until recently, studies of Varroa have been limited by the paucity of genomic tools. Lab- and field-based methods exploiting such methods are still nascent. This study developed a set of methods for preserving Varroa DNA and RNA from the field to the lab and processing them into sequencing libraries. We performed preservation experiments in which Varroa mites were immersed in TRIzol, RNAlater, and absolute ethanol for preservation periods up to 21 days post-treatment to assess DNA and RNA integrity. Results For both DNA and RNA, mites preserved in TRIzol and RNAlater at room temperature degraded within 10 days post-treatment. Mites preserved in ethanol at room temperature and 4 degrees C remained intact through 21 days. Varroa mite DNA and RNA libraries were created and sequenced for ethanol preserved samples, 15 and 21 days post-treatment. All DNA sequences mapped to the V. destructor genome at above 95% on average, while RNA sequences mapped to V. destructor, but also sometimes to high levels of the deformed-wing virus and to various organisms. Conclusions Ethanolic preservation of field-collected mites is inexpensive and simple, and allows them to be shipped and processed successfully in the lab for a wide variety of sequencing applications. It appears to preserve RNA from both Varroa and at least some of the viruses it vectors. |
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| 出版者 | ||||||
| 出版者 | BMC | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1471-2164 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | info:pmid/33446105 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1186/s12864-020-07363-7 | |||||
| 権利 | ||||||
| 権利情報 | © 2020 The Author(s). | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-07363-7 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
