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RNA modifications detection by comparative Nanopore direct RNA sequencing
https://oist.repo.nii.ac.jp/records/2539
https://oist.repo.nii.ac.jp/records/25391b7c209d-212a-4015-8f6f-73bd8a84f68e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
s41467-021-27393-3 (18.0 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2022-01-28 | |||||
| タイトル | ||||||
| タイトル | RNA modifications detection by comparative Nanopore direct RNA sequencing | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 著者（英） |
Leger, Adrien
× Leger, Adrien× Amaral, Paulo P.× Pandolfini, Luca× Capitanchik, Charlotte× Capraro, Federica× Miano, Valentina× Migliori, Valentina× Toolan-Kerr, Patrick× Sideri, Theodora× Enright, Anton J.× Tzelepis, Konstantinos× van Werven, Folkert J.× Luscombe, Nicholas M.× Barbieri, Isaia× Ule, Jernej× Fitzgerald, Tomas× Birney, Ewan× Leonardi, Tommaso× Kouzarides, Tony |
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| 書誌情報 |
en : Nature Communications 巻 12, 号 1, p. 7198, 発行日 2021-10-10 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | RNA molecules undergo a vast array of chemical post-transcriptional modifications (PTMs) that can affect their structure and interaction properties. In recent years, a growing number of PTMs have been successfully mapped to the transcriptome using experimental approaches relying on high-throughput sequencing. Oxford Nanopore direct-RNA sequencing has been shown to be sensitive to RNA modifications. We developed and validated Nanocompore, a robust analytical framework that identifies modifications from these data. Our strategy compares an RNA sample of interest against a non-modified control sample, not requiring a training set and allowing the use of replicates. We show that Nanocompore can detect different RNA modifications with position accuracy in vitro, and we apply it to profile m6A in vivo in yeast and human RNAs, as well as in targeted non-coding RNAs. We confirm our results with orthogonal methods and provide novel insights on the co-occurrence of multiple modified residues on individual RNA molecules. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Nature Portfolio | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | EISSN | |||||
| 収録物識別子 | 2041-1723 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 34893601 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1038/s41467-021-27393-3 | |||||
| 権利 | ||||||
| 権利情報 | © 2021 The Author(s). | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.nature.com/articles/s41467-021-27393-3 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
