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Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage
https://oist.repo.nii.ac.jp/records/2676
https://oist.repo.nii.ac.jp/records/2676542f444b-8099-4b36-9e7f-14f2f96a4995
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
s41467-022-28137-7 (1.8 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2022-07-05 | |||||
| タイトル | ||||||
| タイトル | Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者（英） |
Riesenberg, Stephan
× Riesenberg, Stephan× Helmbrecht, Nelly× Kanis, Philipp× Maricic, Tomislav× Pääbo, Svante |
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| 書誌情報 |
en : Nature Communications 巻 13, 号 1, p. 489, 発行日 2022-01-25 |
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| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The first step in CRISPR-Cas9-mediated genome editing is the cleavage of target DNA sequences that are complementary to so-called spacer sequences in CRISPR guide RNAs (gRNAs). However, some DNA sequences are refractory to CRISPR-Cas9 cleavage, which is at least in part due to gRNA misfolding. To overcome this problem, we have engineered gRNAs with highly stable hairpins in their constant parts and further enhanced their stability by chemical modifications. The ‘Genome-editing Optimized Locked Design’ (GOLD)-gRNA increases genome editing efficiency up to around 1000-fold (from 0.08 to 80.5%) with a mean increase across different other targets of 7.4-fold. We anticipate that this improved gRNA will allow efficient editing regardless of spacer sequence composition and will be especially useful if a desired genomic site is difficult to edit. | |||||
| 出版者 | ||||||
| 出版者 | Nature Portfolio | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2041-1723 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | info:pmid/35078986 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1038/s41467-022-28137-7 | |||||
| 権利 | ||||||
| 権利情報 | © 2022 The Author(s). | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.nature.com/articles/s41467-022-28137-7 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
