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Banp regulates DNA damage response and chromosome segregation during the cell cycle in zebrafish retina
https://oist.repo.nii.ac.jp/records/2759
https://oist.repo.nii.ac.jp/records/2759ce47814c-4f41-4e06-97d1-72e646ff0e35
名前 / ファイル | ライセンス | アクション |
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elife-74611-v1 (14.8 MB)
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CC BY 4.0
Creative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-08-22 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Banp regulates DNA damage response and chromosome segregation during the cell cycle in zebrafish retina | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Babu, Swathy
× Babu, Swathy× Takeuchi, Yuki× Masai, Ichiro |
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書誌情報 |
en : eLife 巻 11, p. e74611, 発行日 2022-08-09 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Btg3-associated nuclear protein (Banp) was originally identified as a nuclear matrix-associated region (MAR)-binding protein and it functions as a tumor suppressor. At the molecular level, Banp regulates transcription of metabolic genes via a CGCG-containing motif called the Banp motif. However, its physiological roles in embryonic development are unknown. Here, we report that Banp is indispensable for the DNA damage response and chromosome segregation during mitosis. Zebrafish banp mutants show mitotic cell accumulation and apoptosis in developing retina. We found that DNA replication stress and tp53-dependent DNA damage responses were activated to induce apoptosis in banp mutants, suggesting that Banp is required for regulation of DNA replication and DNA damage repair. Furthermore, consistent with mitotic cell accumulation, chromosome segregation was not smoothly processed from prometaphase to anaphase in banp morphants, leading to a prolonged M-phase. Our RNA- and ATAC-sequencing identified 31 candidates for direct Banp target genes that carry the Banp motif. Interestingly, a DNA replication fork regulator, wrnip1, and two chromosome segregation regulators, cenpt and ncapg, are included in this list. Thus, Banp directly regulates transcription of wrnip1 for recovery from DNA replication stress, and cenpt and ncapg for chromosome segregation during mitosis. Our findings provide the first in vivo evidence that Banp is required for cell-cycle progression and cell survival by regulating DNA damage responses and chromosome segregation during mitosis. | |||||
出版者 | ||||||
出版者 | eLife Sciences Publications Ltd. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2050-084X | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/35942692 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.7554/eLife.74611 | |||||
権利 | ||||||
権利情報 | © 2022 Babu et al. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://elifesciences.org/articles/74611 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |