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Cryo-electron microscopy for structural analysis of dynamic biological macromolecules
https://oist.repo.nii.ac.jp/records/345
https://oist.repo.nii.ac.jp/records/345367ac13a-f742-4ee6-a468-e07883c85316
名前 / ファイル | ライセンス | アクション |
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1-s2.0-S0304416517302374-main (1.1 MB)
|
Creative Commons Attribution 4.0 International
(http://creativecommons.org/licenses/by/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-03-28 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Cryo-electron microscopy for structural analysis of dynamic biological macromolecules | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Murata, Kazuyoshi
× Murata, Kazuyoshi× Wolf, Matthias |
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書誌情報 |
en : Biochimica et Biophysica Acta (BBA) - General Subjects 巻 1862, 号 2, p. 324-334, 発行日 2017-07-27 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Background: Since the introduction of what became today's standard for cryo-embedding of biological macromolecules at native conditions more than 30 years ago, techniques and equipment have been drastically improved and the structure of biomolecules can now be studied at near atomic resolution by cryo-electron microscopy (cryo-EM) while capturing multiple dynamic states. Here we review the recent progress in cryo-EM for structural studies of dynamic biological macromolecules. Scope of review: We provide an overview of the cryo-EM method and introduce contemporary studies to investigate biomolecular structure and dynamics, including examples from the recent literature. Major conclusions: Cryo-EM is a powerful tool for the investigation of biological macromolecular structures including analysis of their dynamics by using advanced image-processing algorithms. The method has become even more widely applicable with present-day single particle analysis and electron tomography. General significance: The cryo-EM method can be used to determine the three-dimensional structure of biomacromolecules in near native condition at close to atomic resolution, and has the potential to reveal conformations of dynamic molecular complexes. This article is part of a Special Issue entitled "Biophysical Exploration of Dynamical Ordering of Biomolecular Systems" edited by Dr. Koichi Kato. |
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出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0304-4165 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/28756276 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.bbagen.2017.07.020 | |||||
権利 | ||||||
権利情報 | © 2017 The Authors | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.sciencedirect.com/science/article/pii/S0304416517302374?via%3Dihub | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |