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A Streamlined Method for the Preparation of Gelatin Embedded Brains and Simplified Organization of Sections for Serial Reconstructions
https://oist.repo.nii.ac.jp/records/410
https://oist.repo.nii.ac.jp/records/4103eac8d88-0375-4772-bb0e-9767655de872
名前 / ファイル | ライセンス | アクション |
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Bio-protocol2610 (3.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-07-03 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | A Streamlined Method for the Preparation of Gelatin Embedded Brains and Simplified Organization of Sections for Serial Reconstructions | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Liu, Andrew
× Liu, Andrew× Aoki, Sho× Wickens, Jeffery |
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書誌情報 |
en : BIO-PROTOCOL 巻 7, 号 22, p. e2610, 発行日 2017-11-20 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Gelatin embedding of whole brains for sectioning is a critical procedure used in neuroscience to ensure all morphological and spatial details are preserved intact. Here, we describe an inexpensive, reproducible and efficient means to embed post-fixed brains ready for sectioning in gelatin within a week’s time. The sections obtained are distortion-free and their fragile internal structures preserved which can be used for serial reconstructions for lesion studies and mapping of viral expression after stereotaxic injections. In addition, the separation of adjacent slices into a series of 3-4 vials facilitates subsequent organization and assembly of serial sections at the mounting step. | |||||
出版者 | ||||||
出版者 | Bio-protocol | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2331-8325 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.21769/BioProtoc.2610 | |||||
権利 | ||||||
権利情報 | © 2017 The Authors; exclusive licensee Bio-protocol LLC. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://bio-protocol.org/e2610 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |