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Firefly Luciferase Mutant with Enhanced Activity and Thermostability
https://oist.repo.nii.ac.jp/records/727
https://oist.repo.nii.ac.jp/records/7271ab19c0c-55da-4673-b2a6-631ddff56d58
名前 / ファイル | ライセンス | アクション |
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acsomega.7b02068 (2.7 MB)
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ACS AuthorChoice
(https://pubs.acs.org/page/policy/authorchoice/index.html) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-10-22 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Firefly Luciferase Mutant with Enhanced Activity and Thermostability | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Pozzo, Tania
× Pozzo, Tania× Akter, Farhima× Nomura, Yoko× Louie, Angelique Y.× Yokobayashi, Yohei |
|||||
書誌情報 |
en : ACS Omega 巻 3, 号 3, p. 2628-2633, 発行日 2018-03-06 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The luciferase isolated from the firefly Photinus pyralis (Ppy) catalyzes a two-step reaction that results in the oxidation of d-luciferin accompanied by emission of yellow-green light with a peak at 560 nm. Among many applications, Ppy luciferase has been used extensively as a reporter gene in living cells and organisms. However, some biological applications are limited by the low stability of the luciferase and limited intracellular luciferin concentration. To address these challenges, efforts to protein engineer Ppy luciferase have resulted in a number of mutants with improved properties such as thermostability, pH tolerance, and catalytic turn over. In this work, we combined amino acid mutations that were shown to enhance the enzyme's thermostability (Mutant E) with those reported to enhance catalytic activity (LGR). The resulting mutant (YY5) contained eight amino acid changes from the wild-type luciferase and exhibited both improved thermostability and brighter luminescence at low luciferin concentrations. Therefore, YY5 may be useful for reporter gene applications. | |||||
出版者 | ||||||
出版者 | American Chemical Society | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2470-1343 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/30023842 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1021/acsomega.7b02068 | |||||
権利 | ||||||
権利情報 | © 2018 American Chemical Society | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://pubs.acs.org/doi/10.1021/acsomega.7b02068 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |