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Optogenetic induction of hibernation-like state with modified human Opsin4 in mice
https://oist.repo.nii.ac.jp/records/2848
https://oist.repo.nii.ac.jp/records/2848727daf26-0501-4ed6-9215-733a46b548a5
名前 / ファイル | ライセンス | アクション |
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1-s2.0-S2667237522002338-main (24.6 MB)
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CC BY 4.0
Creative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-11-17 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Optogenetic induction of hibernation-like state with modified human Opsin4 in mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | optogenetics | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | OPN4 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hibernation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | torpor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | fiber-less optogenetics | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | melanopsin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | QRFP | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | body temperature | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | GPCR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | neuroscience | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Takahashi, Tohru M.
× Takahashi, Tohru M.× Hirano, Arisa× Kanda, Takeshi× Saito, Viviane M.× Ashitomi, Hiroto× Tanaka, Kazumasa Z.× Yokoshiki, Yasufumi× Masuda, Kosaku× Yanagisawa, Masashi× Vogt, Kaspar E.× Tokuda, Takashi× Sakurai, Takeshi |
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書誌情報 |
en : Cell Reports Methods 巻 2, 号 11, p. 100336, 発行日 2022-11-21 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | We recently determined that the excitatory manipulation of Qrfp-expressing neurons in the preoptic area of the hypothalamus (quiescence-inducing neurons [Q neurons]) induced a hibernation-like hypothermic/hypometabolic state (QIH) in mice. To control the QIH with a higher time resolution, we develop an optogenetic method using modified human opsin4 (OPN4; also known as melanopsin), a G protein-coupled-receptor-type blue-light photoreceptor. C-terminally truncated OPN4 (OPN4dC) stably and reproducibly induces QIH for at least 24 h by illumination with low-power light (3 μW, 473 nm laser) with high temporal resolution. The high sensitivity of OPN4dC allows us to transcranially stimulate Q neurons with blue-light-emitting diodes and non-invasively induce the QIH. OPN4dC-mediated QIH recapitulates the kinetics of the physiological changes observed in natural hibernation, revealing that Q neurons concurrently contribute to thermoregulation and cardiovascular function. This optogenetic method may facilitate identification of the neural mechanisms underlying long-term dormancy states such as sleep, daily torpor, and hibernation. | |||||
出版者 | ||||||
出版者 | Cell Press | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2667-2375 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.crmeth.2022.100336 | |||||
権利 | ||||||
権利情報 | © 2022 The Author(s) | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.sciencedirect.com/science/article/pii/S2667237522002338 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |