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Spatiotemporally tracking of nano-biofilaments inside the nuclear pore complex core
https://oist.repo.nii.ac.jp/records/1700
https://oist.repo.nii.ac.jp/records/1700b671dc63-1816-4555-8ccb-c6cd41156d71
名前 / ファイル | ライセンス | アクション |
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1-s2.0-S0142961220304440-main (3.4 MB)
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International(https://creativecommons.org/licenses/by-nc-nd/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-09-24 | |||||
タイトル | ||||||
タイトル | Spatiotemporally tracking of nano-biofilaments inside the nuclear pore complex core | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Mohamed, Mahmoud Shaaban
× Mohamed, Mahmoud Shaaban× Hazawa, Masaharu× Kobayashi, Akiko× Guillaud, Laurent× Watanabe-Nakayama, Takahiro× Nakayama, Mizuho× Wang, Hanbo× Kodera, Noriyuki× Oshima, Masanobu× Ando, Toshio× Wong, Richard W. |
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書誌情報 |
en : Biomaterials 巻 256, p. 120198, 発行日 2020-06-23 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nuclear pore complex (NPC) is a gating nanomachine with a central selective barrier composed mainly of Nups, which contain intrinsically disordered (non-structured) regions (IDRs) with phenylalanine-glycine (FG) motifs (FG-NUPs). The NPC central FG network dynamics is poorly understood, as FG-NUPs liquid-liquid phase separation (LLPS) have evaded structural characterization. Moreover, the working mechanism of single FG-NUP-biofilaments residing at the central lumen is unknown. In general, flexible biofilaments are expected to be tangled and knotted during their motion and interaction. However, filament knotting visualization in real-time and space has yet to be visualized at the nanoscale. Here, we report a spatiotemporally tracking method for FG-NUP organization with nanoscale resolution, unveiling FG-NUP conformation in NPCs of colorectal cells and organoids at timescales of ~150 ms using high-speed atomic force microscopy (HS-AFM). Tracking of FG-NUP single filaments revealed that single filaments have a heterogeneous thickness in normal and cancer models which in turn affected the filament rotation and motion. Notably, FG-NUPs are overexpressed in various cancers. Using the FG-NUP inhibitor, trans-1,2-cyclohexanediol, we found that central plug size was significantly reduced and incompletely reversible back to filamentous structures in aggressive colon cancer cells and organoids. These data showed a model of FG-NUPs reversible self-assembly devolving into the central plug partial biogenesis. Taken together, HS-AFM enabled the tracking and manipulation of single filaments of native FG-NUPs which has remained evasive for decades. | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0142-9612 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/32622019 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.biomaterials.2020.120198 | |||||
権利 | ||||||
権利情報 | © 2020 The Authors. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.sciencedirect.com/science/article/pii/S0142961220304440 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |