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マウス運動前野におけるドーパミン、ノルアドレナリン、セロトニンの時空間応答の2光子イメージング
https://doi.org/10.15102/0002000518
https://doi.org/10.15102/00020005180d673413-af7c-44e7-8814-1a0413dfbc34
| 名前 / ファイル | ライセンス | アクション |
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DesforgesMilesFulltext.pdf (42.1 MB)
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DesforgesMilesExamAbstract.pdf (48 KB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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| 公開日 | 2024-06-04 | |||||||
| タイトル | ||||||||
| タイトル | マウス運動前野におけるドーパミン、ノルアドレナリン、セロトニンの時空間応答の2光子イメージング | |||||||
| 言語 | ja | |||||||
| タイトル | ||||||||
| タイトル | Characterisation of Serotonin, Noradrenaline and Dopamine Release in the Motor Cortex | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Behavioral neuroscience | Neurophysiology, especially neuromodulators | Two-photon imaging | Neuromodulator biosensors | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| ID登録 | ||||||||
| ID登録 | 10.15102/0002000518 | |||||||
| ID登録タイプ | JaLC | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 著者 (英) |
Desforges, Miles
× Desforges, Miles
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | The precise spatiotemporal dynamics of neuromodulator release in the cortex remain elusive. These release patterns determine receptor activation, as receptors with different affinities are activated by different concentrations, and thereby distances, from the release site. While neuromodulator nuclei have been extensively characterised, technological challenges have hindered detailed cortical investigation. This thesis clarifies the release patterns of dopamine, noradrenaline, and serotonin in the secondary motor cortex (M2) using a novel orthogonalized Go/NoGo task developed for head-fixed mice. We dissect neuromodulator activity at the intersection of locomotion and unconditioned stimuli (US). Employing two photon microscopy and novel genetically encoded sensors, we achieved high-resolution imaging unattainable with previous techniques. Our findings indicate that serotonin, noradrenaline, and dopamine are robustly correlated with locomotion transitions, exhibiting significant increases from rest to locomotion and decreases during the reverse. Cross-correlation analysis suggests neuromodulator activity precedes locomotion onset, potentially facilitating motor behaviours. Unexpectedly, both appetitive (sucrose) and aversive (air-puff) stimuli elicited fluorescence decreases in all neuromodulators. Serotonin and noradrenaline showed stronger, more consistent responses during movement than rest, while dopamine responses were more consistent across locomotion states. Detailed analysis of activity maxima and minima within individual trials revealed significant variability and complexity. Contrary to expectations, no neuromodulator responses to US-predicting cues were observed, despite anticipatory behavioural changes. This highlights the need for further investigation into sensory cue processing in M2. Our findings suggest a complex multiplexing of information in M2, whereby neuromodulator activity is more influenced by locomotive state than appetitive or aversive stimuli delivery. | |||||||
| 言語 | en | |||||||
| 口頭試問日 | ||||||||
| 値 | 2024-02-29 | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2024-05-31 | |||||||
| 学位名 | ||||||||
| 学位名 | Doctor of Philosophy | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲第153号 | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||
| 学位授与機関識別子 | 38005 | |||||||
| 学位授与機関名 | Okinawa Institute of Science and Technology Graduate University | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 権利 | ||||||||
| 権利情報 | © 2024 The Author. | |||||||
