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Ultrastructure of influenza virus ribonucleoprotein complexes during viral RNA synthesis
https://oist.repo.nii.ac.jp/records/2237
https://oist.repo.nii.ac.jp/records/22376c522b5e-af19-4565-ad38-6c8bb09b4929
名前 / ファイル | ライセンス | アクション |
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Nakano-2021-Ultrastructure of influenza virus (2.2 MB)
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CC BY 4.0
Creative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-09-07 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Ultrastructure of influenza virus ribonucleoprotein complexes during viral RNA synthesis | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | CRYSTAL-STRUCTURE | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | STRUCTURAL BASIS | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | MESSENGER-RNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | CAP-BINDING | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | VIRION RNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | POLYMERASE | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ENDONUCLEASE | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | POLYADENYLATION | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DYNAMICS | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | INSIGHTS | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Nakano, Masahiro
× Nakano, Masahiro× Sugita, Yukihiko× Kodera, Noriyuki× Miyamoto, Sho× Muramoto, Yukiko× Wolf, Matthias× Noda, Takeshi |
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書誌情報 |
en : Communications Biology 巻 4, 号 858, 発行日 2021-07-09 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The single-stranded, negative-sense, viral genomic RNA (vRNA) of influenza A virus is encapsidated by viral nucleoproteins (NPs) and an RNA polymerase to form a ribonucleoprotein complex (vRNP) with a helical, rod-shaped structure. The vRNP is responsible for transcription and replication of the vRNA. However, the vRNP conformation during RNA synthesis is not well understood. Here, using high-speed atomic force microscopy and cryo-electron microscopy, we investigated the native structure of influenza A vRNPs during RNA synthesis in vitro. Two distinct types of vRNPs were observed in association with newly synthesized RNAs: an intact, helical rod-shaped vRNP connected with a folded RNA and a deformed vRNP associated with a looped RNA. Interestingly, the looped RNA was a double-stranded RNA, which likely comprises a nascent RNA and the template RNA detached from NPs of the vRNP. These results suggest that while some vRNPs keep their helical structures during RNA synthesis, for the repeated cycle of RNA synthesis, others accidentally become structurally deformed, which likely results in failure to commence or continue RNA synthesis. Thus, our findings provide the ultrastructural feature of vRNPs during RNA synthesis. | |||||
出版者 | ||||||
出版者 | Nature Portfolio | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2399-3642 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/34244608 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1038/s42003-021-02388-4 | |||||
権利 | ||||||
権利情報 | © 2021 The Author(s) | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.nature.com/articles/s42003-021-02388-4 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |