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Efficient Exploration of Sequence Space by Sequence-Guided Protein Engineering and Design
https://oist.repo.nii.ac.jp/records/2724
https://oist.repo.nii.ac.jp/records/2724a99fd67c-720f-4b62-8cf5-319ef003a8d6
| 名前 / ファイル | ライセンス | アクション |
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20220206 Biochemistry review revision - revis (909.8 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2022-08-01 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Efficient Exploration of Sequence Space by Sequence-Guided Protein Engineering and Design | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者（英） |
Clifton, Ben E.
× Clifton, Ben E.
× Kozome, Dan
× Laurino, Paola
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| 書誌情報 |
en : Biochemistry 発行日 2022-03-04 |
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| 抄録 | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | The rapid growth of sequence databases over the past two decades means that protein engineers faced with optimizing a protein for any given task will often have immediate access to a vast number of related protein sequences. These sequences encode information about the evolutionary history of the protein and the underlying sequence requirements to produce folded, stable, and functional protein variants. Methods that can take advantage of this information are an increasingly important part of the protein engineering tool kit. In this Perspective, we discuss the utility of sequence data in protein engineering and design, focusing on recent advances in three main areas: the use of ancestral sequence reconstruction as an engineering tool to generate thermostable and multifunctional proteins, the use of sequence data to guide engineering of multipoint mutants by structure-based computational protein design, and the use of unlabeled sequence data for unsupervised and semisupervised machine learning, allowing the generation of diverse and functional protein sequences in unexplored regions of sequence space. Altogether, these methods enable the rapid exploration of sequence space within regions enriched with functional proteins and therefore have great potential for accelerating the engineering of stable, functional, and diverse proteins for industrial and biomedical applications. | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | American Chemical Society | |||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 0006-2960 | |||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 1520-4995 | |||||||||||
| PubMed番号 | ||||||||||||
| 関連タイプ | isVersionOf | |||||||||||
| 識別子タイプ | PMID | |||||||||||
| 関連識別子 | info:pmid/35245020 | |||||||||||
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| 関連タイプ | isVersionOf | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | info:doi/10.1021/acs.biochem.1c00757 | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | © 2022 The Author(s) | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Biochemistry, copyright © 2022 The Author(s) after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.biochem.1c00757 | |||||||||||
| 関連サイト | ||||||||||||
| 識別子タイプ | URI | |||||||||||
| 関連識別子 | https://pubs.acs.org/doi/10.1021/acs.biochem.1c00757 | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | AM | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||
