{"_buckets": {"deposit": "2d202c30-edd8-4250-bd7d-f272d66f55fb"}, "_deposit": {"created_by": 26, "id": "460", "owners": [26], "pid": {"revision_id": 0, "type": "depid", "value": "460"}, "status": "published"}, "_oai": {"id": "oai:oist.repo.nii.ac.jp:00000460", "sets": ["44"]}, "author_link": ["1900", "1896", "1901", "1899", "1897", "1898"], "item_10001_biblio_info_7": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-09-27", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "10", "bibliographicPageEnd": "2649", "bibliographicPageStart": "2640", "bibliographicVolumeNumber": "9", "bibliographic_titles": [{}, {"bibliographic_title": "Genome Biology and Evolution", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "Author", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Aird, Steven D."}], "nameIdentifiers": [{"nameIdentifier": "1896", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Arora, Jigyasa"}], "nameIdentifiers": [{"nameIdentifier": "1897", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Barua, Agneesh"}], "nameIdentifiers": [{"nameIdentifier": "1898", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Qiu, Lijun"}], "nameIdentifiers": [{"nameIdentifier": "1899", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Terada, Kouki"}], "nameIdentifiers": [{"nameIdentifier": "1900", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mikheyev, Alexander S."}], "nameIdentifiers": [{"nameIdentifier": "1901", "nameIdentifierScheme": "WEKO"}]}]}, "item_10001_description_5": {"attribute_name": "Abstract", "attribute_value_mlt": [{"subitem_description": "Venoms are among the most biologically active secretions known, and are commonly believed to evolve under extreme positive selection. Many venom gene families, however, have undergone duplication, and are often deployed in doses vastly exceeding the LD50 for most prey species, which should reduce the strength of positive selection. Here, we contrast these selective regimes using snake venoms, which consist of rapidly evolving protein formulations. Though decades of extensive studies have found that snake venom proteins are subject to strong positive selection, the greater action of drift has been hypothesized, but never tested. Using a combination of de novo genome sequencing, population genomics, transcriptomics, and proteomics, we compare the two modes of evolution in the pitviper, Protobothrops mucrosquamatus. By partitioning selective constraints and adaptive evolution in a McDonald–Kreitman-type framework, we find support for both hypotheses: venom proteins indeed experience both stronger positive selection, and lower selective constraint than other genes in the genome. Furthermore, the strength of selection may be modulated by expression level, with more abundant proteins experiencing weaker selective constraint, leading to the accumulation of more deleterious mutations. These findings show that snake venoms evolve by a combination of adaptive and neutral mechanisms, both of which explain their extraordinarily high rates of molecular evolution. In addition to positive selection, which optimizes efficacy of the venom in the short term, relaxed selective constraints for deleterious mutations can lead to more rapid turnover of individual proteins, and potentially to exploration of a larger venom phenotypic space.", "subitem_description_type": "Other"}]}, "item_10001_publisher_8": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_publisher": "Oxford University Press"}, {"subitem_publisher": "Society for Molecular Biology and Evolution"}]}, "item_10001_relation_13": {"attribute_name": "PubMedNo.", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:pmid/29048530", "subitem_relation_type_select": "PMID"}}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1093/gbe/evx199", "subitem_relation_type_select": "DOI"}}]}, "item_10001_relation_17": {"attribute_name": "Related site", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://academic.oup.com/gbe/article/9/10/2640/4259791", "subitem_relation_type_select": "URI"}}]}, "item_10001_rights_15": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_rights": " © 2017 The Author(s)."}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1759-6653", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_version_type_20": {"attribute_name": "Author\u0027s flag", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-07-27"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "evx199.pdf", "filesize": [{"value": "371.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "CreativeCommonsAttributionNon-CommercialLicense\n(http://creativecommons.org/licenses/by-nc/4.0/)", "licensetype": "license_free", "mimetype": "application/pdf", "size": 371000.0, "url": {"label": "evx199", "url": "https://oist.repo.nii.ac.jp/record/460/files/evx199.pdf"}, "version_id": "5374ee9b-6d7f-4bf5-950e-bca9240f432d"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Population Genomic Analysis of a Pitviper Reveals Microevolutionary Forces Underlying Venom Chemistry", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Population Genomic Analysis of a Pitviper Reveals Microevolutionary Forces Underlying Venom Chemistry", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "26", "path": ["44"], "permalink_uri": "https://oist.repo.nii.ac.jp/records/460", "pubdate": {"attribute_name": "公開日", "attribute_value": "2018-07-27"}, "publish_date": "2018-07-27", "publish_status": "0", "recid": "460", "relation": {}, "relation_version_is_last": true, "title": ["Population Genomic Analysis of a Pitviper Reveals Microevolutionary Forces Underlying Venom Chemistry"], "weko_shared_id": 26}