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TDP-43 condensation properties specify its RNA-binding and regulatory repertoire
https://oist.repo.nii.ac.jp/records/2291
https://oist.repo.nii.ac.jp/records/229143f90ba1-acbd-4ac0-8ee1-ed811f1fbc09
名前 / ファイル | ライセンス | アクション |
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1-s2.0-S0092867421008771-main (10.9 MB)
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CC BY 4.0
Creative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-09-17 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | TDP-43 condensation properties specify its RNA-binding and regulatory repertoire | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | RNA-binding protei | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | TDP-43 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | iCLIP | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | condensation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | RNA granules | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | phase separation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | intrinsically disordered region | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | amyotrophic lateral sclerosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | multivalency | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | alternative polyadenylation | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Hallegger, Martina
× Hallegger, Martina× Chakrabarti, Anob M.× Lee, Flora C.Y.× Lee, Bo Lim× Amalietti, Aram G.× Odeh, Hana M.× Copley, Katie E.× Rubien, Jack D.× Portz, Bede× Kuret, Klara× Huppertz, Ina× Rau, Frédérique× Patani, Rickie× Fawzi, Nicolas L.× Shorter, James× Luscombe, Nicholas M.× Ule, Jernej |
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書誌情報 |
en : Cell 巻 184, 号 18, p. 4680-4696.e22, 発行日 2021-09-02 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Mutations causing amyotrophic lateral sclerosis (ALS) often affect the condensation properties of RNA-binding proteins (RBPs). However, the role of RBP condensation in the specificity and function of protein-RNA complexes remains unclear. We created a series of TDP-43 C-terminal domain (CTD) variants that exhibited a gradient of low to high condensation propensity, as observed in vitro and by nuclear mobility and foci formation. Notably, a capacity for condensation was required for efficient TDP-43 assembly on subsets of RNA-binding regions, which contain unusually long clusters of motifs of characteristic types and density. These “binding-region condensates” are promoted by homomeric CTD-driven interactions and required for efficient regulation of a subset of bound transcripts, including autoregulation of TDP-43 mRNA. We establish that RBP condensation can occur in a binding-region-specific manner to selectively modulate transcriptome-wide RNA regulation, which has implications for remodeling RNA networks in the context of signaling, disease, and evolution. | |||||
出版者 | ||||||
出版者 | Elsevier Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0092-8674 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1097-4172 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/34380047 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.cell.2021.07.018 | |||||
権利 | ||||||
権利情報 | © 2021 The Author(s) | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.sciencedirect.com/science/article/pii/S0092867421008771 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |